验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293T cells, HeLa cells, HepG2 cells, Jurkat cells, MCF-7 cells, mouse brain tissue, rat brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
55409-1-AP targets KHSRP in WB, IF, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IF, RIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | KH-type splicing regulatory protein |
| 别名 | FBP2, FUBP2, FUSE binding protein 2, KHSRP, KSRP, p75 |
| 计算分子量 | 73 kDa |
| 观测分子量 | 75 kDa |
| GenBank蛋白编号 | NM_003685 |
| 基因名称 | KHSRP |
| Gene ID (NCBI) | 8570 |
| RRID | AB_11182170 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q92945 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
KHSRP, also named as FUBP2, KSRP, and p75, belongs to the KHSRP family. It binds to the dendritic targeting element and may play a role in mRNA trafficking. KHSRP is a part of a ternary complex that binds to the downstream control sequence (DCS) of the pre-mRNA. It mediates exon inclusion in transcripts that are subject to tissue-specific alternative splicing. KHSRP may interact with single-stranded DNA from the far-upstream element (FUSE). May activate gene expression. It is involved in the degradation of inherently unstable mRNAs that contain AU-rich elements (AREs) in their 3'-UTR, possibly by recruiting degradation machinery to ARE-containing mRNAs.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for KHSRP antibody 55409-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nucleic Acids Res G3BP1 binds to guanine quadruplexes in mRNAs to modulate their stabilities. | ||
iScience Mutational burden of XPNPEP3 leads to defects in mitochondrial complex I and cilia in NPHPL1 | ||
Atherosclerosis microRNA-185 modulates low density lipoprotein receptor expression as a key posttranscriptional regulator. | ||
Int Immunopharmacol Angiotensin II contributes to intratumoral immunosuppressionvia induction of PD-L1 expression in non-small cell lung carcinoma.
| ||
Cell Rep Identification of cis-HOX-HOXC10 axis as a therapeutic target for colorectal tumor-initiating cells without APC mutations.
| ||
Biochim Biophys Acta Subcellular quantitative proteomic analysis reveals host proteins involved in human cytomegalovirus infection. |
