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验证数据展示

  • IF Staining of rat eye using 83272-1-RR (same clone as 83272-1-PBS)

    IF Staining of rat eye using 83272-1-RR (same clone as 83272-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat eye tissue using KERA antibody (83272-1-RR, Clone: 230399B11 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83272-1-PBS in a different storage buffer formulation.

  • FC experiment of U251 using 83272-1-RR (same clone as 83272-1-PBS)

    FC experiment of U251 using 83272-1-RR (same clone as 83272-1-PBS)

    1x10^6 U251 were intracellularly stained with 0.2 ug KERA Recombinant Antibody (83272-1-RR, Clone:230399B11) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.2 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83272-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83272-1-RR

    Affinity and Kinetic Characterization of 83272-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83272-1-RR against Human KERA were performed. The affinity constant is below 1 pM.

KERA Recombinant antibody, PBS Only

KERA Recombinant Antibody for IF-P, FC (Intra), Indirect ELISA
Cat No. 83272-1-PBS

产品说明书

CloneNo. 230399B11

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF-P, FC (Intra), Indirect ELISA

别名

Keratocan, 230399B11, CNA2, KTN, SLRR2B

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

83272-1-PBS targets KERA in IF-P, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 IF-P, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原 KERA fusion protein Ag34171 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 keratocan
别名 Keratocan, 230399B11, CNA2, KTN, SLRR2B
计算分子量 352 aa, 41 kDa
GenBank蛋白编号BC032667
基因名称 KERA
Gene ID (NCBI) 11081
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDO60938
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Keratocan and Lumican are the predominant class II SLRPs(Small leucine-rich proteoglycans) in the corneal stroma. While not totally corneal-specific together these SLRPs are excellent biomarkers for the tissue. Mutant mice deficient for keratocan expression have transparent but thin corneal stromas with a mild increase in collagen fibril diameter with no further abnormalities.In humans, mutations in the keratocan gene are associated with a flattened cornea in a rare condition known as cornea plana. The relative specificity of keratocan to corneal keratocytes has been useful for tissue-specific targeting for genetic manipulation of the corneal stroma.(PMID: 32663498)