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验证数据展示

  • WB analysis of human placenta using 16228-1-AP

    WB analysis of human placenta using 16228-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 16228-1-AP (KDSR antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 16228-1-AP

    IHC staining of human placenta using 16228-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 16228-1-AP (KDSR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human placenta using 16228-1-AP

    IHC staining of human placenta using 16228-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 16228-1-AP (KDSR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

KDSR Polyclonal antibody

KDSR Polyclonal Antibody for WB, IHC, ELISA
Cat No. 16228-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

3 dehydrosphinganine reductase, 3-dehydrosphinganine reductase, 3-ketodihydrosphingosine reductase, DHSR, EC:1.1.1.102

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inhuman placenta tissue
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

16228-1-AP targets KDSR in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原

CatNo: Ag8176

Product name: Recombinant human KDSR protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 51-332 aa of BC008797

Sequence: ECYKQGAFITLVARNEDKLLQAKKEIEMHSINDKQVVLCISVDVSQDYNQVENVIKQAQEKLGPVDMLVNCAGMAVSGKFEDLEVSTFERLMSINYLGSVYPSRAVITTMKERRVGRIVFVSSQAGQLGLFGFTAYSASKFAIRGLAEALQMEVKPYNVYITVAYPPDTDTPGFAEENRTKPLETRLISETTSVCKPEQVAKQIVKDAIQGNFNSSLGSDGYMLSALTCGMAPVTSITEGLQQVVTMGLFRTIALFYLGSFDSIVRRCMMQREKSENADKTA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 3-ketodihydrosphingosine reductase
别名 3 dehydrosphinganine reductase, 3-dehydrosphinganine reductase, 3-ketodihydrosphingosine reductase, DHSR, EC:1.1.1.102
计算分子量 36 kDa
观测分子量 36 kDa
GenBank蛋白编号BC008797
基因名称 KDSR
Gene ID (NCBI) 2531
RRIDAB_2249648
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ06136
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for KDSR antibody 16228-1-APDownload protocol
IHC protocol for KDSR antibody 16228-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Rep

Aberrant regulation of serine metabolism drives extracellular vesicle release and cancer progression

Authors - Tomofumi Yamamoto
View Article