验证数据展示
经过测试的应用
| Positive WB detected in | PC-3 cells |
| Positive IHC detected in | human thymus tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
22591-1-AP targets KDM4D in WB, IP, IHC, ChIP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IP, ChIP |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | KDM4D fusion protein Ag18436 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | lysine (K)-specific demethylase 4D |
| 别名 | JHDM3D, JMJD2D, KDM4D, lysine (K) demethylase 4D, Lysine specific demethylase 4D |
| 计算分子量 | 523 aa, 59 kDa |
| 观测分子量 | 59 kDa |
| GenBank蛋白编号 | BC122858 |
| 基因名称 | KDM4D |
| Gene ID (NCBI) | 55693 |
| RRID | AB_2879130 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q6B0I6 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for KDM4D antibody 22591-1-AP | Download protocol |
| IHC protocol for KDM4D antibody 22591-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
JHEP Rep JMJD2D stabilises and cooperates with HBx protein to promote HBV transcription and replication
| ||
Front Cell Dev Biol A KDM4-DBC1-SIRT1 Axis Contributes to TGF-b Induced Mesenchymal Transition of Intestinal Epithelial Cells |
