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KDM4D Polyclonal antibody

KDM4D Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

IHC, WB,ELISA and More (2)

Conjugate

Unconjugated

Cat No : 22591-1-AP

Print datasheet

Synonyms

JHDM3D, JMJD2D, KDM4D, lysine (K) demethylase 4D, Lysine specific demethylase 4D

验证数据展示

  • WB analysis of PC-3 using 22591-1-AP

    WB analysis of PC-3 using 22591-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 22591-1-AP (KDM4D Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human thymus using 22591-1-AP

    IHC staining of human thymus using 22591-1-AP

    Immunohistochemical analysis of paraffin-embedded human thymus tissue slide using 22591-1-AP (KDM4D Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inPC-3 cells
Positive IHC detected inhuman thymus tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 22591-1-AP is KDM4D Fusion Protein expressed in E. coli.

Tested Applications IHC, WB,ELISA
Cited ApplicationsWB, IP, ChIP
Tested Reactivity human
Cited Reactivityhuman, mouse, rat
Immunogen KDM4D fusion protein Ag18436 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name lysine (K)-specific demethylase 4D
Synonyms JHDM3D, JMJD2D, KDM4D, lysine (K) demethylase 4D, Lysine specific demethylase 4D
Calculated Molecular Weight 523 aa, 59 kDa
Observed Molecular Weight 59 kDa
GenBank Accession NumberBC122858
Gene Symbol KDM4D
Gene ID (NCBI) 55693
RRIDAB_2879130
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6B0I6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for KDM4D antibody 22591-1-APDownload protocol
IHC protocol for KDM4D antibody 22591-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouse,humanWB,IP

JHEP Rep

JMJD2D stabilises and cooperates with HBx protein to promote HBV transcription and replication

Authors - Xu Kong
  • KD Validated
View Article
ratChIP

Front Cell Dev Biol

A KDM4-DBC1-SIRT1 Axis Contributes to TGF-b Induced Mesenchymal Transition of Intestinal Epithelial Cells

Authors - Baoyu Chen
View Article