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KDM3B Polyclonal antibody

KDM3B Polyclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, WB, ELISA

Conjugate

Unconjugated

Cat No : 19915-1-AP

Print datasheet

Synonyms

5qNCA, C5orf7, JHDM2B, JMJD1B, KDM3B, KIAA1082, lysine (K) demethylase 3B, Lysine specific demethylase 3B, NET22, Nuclear protein 5qNCA

验证数据展示

  • WB analysis using 19915-1-AP

    WB analysis using 19915-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19915-1-AP (KDM3B antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 19915-1-AP

    IF Staining of A431 using 19915-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using KDM3B antibody (19915-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inHEK-293 cells, human placenta tissue, HeLa cells, HepG2 cells
Positive IF detected inA431 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

19915-1-AP targets KDM3B in WB, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen KDM3B fusion protein Ag13795 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name lysine (K)-specific demethylase 3B
Synonyms 5qNCA, C5orf7, JHDM2B, JMJD1B, KDM3B, KIAA1082, lysine (K) demethylase 3B, Lysine specific demethylase 3B, NET22, Nuclear protein 5qNCA
Calculated Molecular Weight 1761 aa, 192 kDa
Observed Molecular Weight 192 kDa, 85 kDa
GenBank Accession NumberBC001202
Gene Symbol KDM3B
Gene ID (NCBI) 51780
RRIDAB_10693529
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7LBC6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for KDM3B antibody 19915-1-APDownload protocol
IF protocol for KDM3B antibody 19915-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Genet

N6-Methyladenosine co-transcriptionally directs the demethylation of histone H3K9me2.

Authors - Yuan Li
View Article