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验证数据展示

  • WB analysis of Y79 using 13443-1-AP

    WB analysis of Y79 using 13443-1-AP

    Y79 cells were subjected to SDS PAGE followed by western blot with 13443-1-AP (KCNS2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human eye using 13443-1-AP

    IHC staining of human eye using 13443-1-AP

    Immunohistochemical analysis of paraffin-embedded human eye using 13443-1-AP (KCNS2 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human eye using 13443-1-AP

    IHC staining of human eye using 13443-1-AP

    Immunohistochemical analysis of paraffin-embedded human eye using 13443-1-AP (KCNS2 antibody) at dilution of 1:100 (under 40x lens).

  • IP experiment of HepG2 using 13443-1-AP

    IP experiment of HepG2 using 13443-1-AP

    IP result of anti-KCNS2 (IP:13443-1-AP, 4ug; Detection:13443-1-AP 1:300) with HepG2 cells lysate 3400ug.

KCNS2 Polyclonal antibody

KCNS2 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 13443-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, IHC, ELISA

别名

KCNS2, KIAA1144, KV9.2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inY79 cells, HepG2 cells, mouse brain tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman eye tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13443-1-AP targets KCNS2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag4271

Product name: Recombinant human KCNS2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-187 aa of BC027932

Sequence: MTGQSLWDVSEANVEDGEIRINVGGFKRRLRSHTLLRFPETRLGRLLLCHSREAILELCDDYDDVQREFYFDRNPELFPYVLHFYHTGKLHVMAELCVFSFSQEIEYWGINEFFIDSCCSYSYHGRKVEPEQEKWDEQSDQESTTSSFDEILAFYNDASKFDGQPLGNFRRQLWLALDNPGYSVLSR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 potassium voltage-gated channel, delayed-rectifier, subfamily S, member 2
别名 KCNS2, KIAA1144, KV9.2
计算分子量 477 aa, 54 kDa
观测分子量 65-70 kDa
GenBank蛋白编号BC027932
基因名称 KCNS2
Gene ID (NCBI) 3788
RRIDAB_2249596
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9ULS6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for KCNS2 antibody 13443-1-APDownload protocol
IHC protocol for KCNS2 antibody 13443-1-APDownload protocol
IP protocol for KCNS2 antibody 13443-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols