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验证数据展示

  • WB analysis using 28000-1-AP

    WB analysis using 28000-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28000-1-AP (KCNQ5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IP experiment of Raji using 28000-1-AP

    IP experiment of Raji using 28000-1-AP

    IP result of anti-KCNQ5 (IP:28000-1-AP, 4ug; Detection:28000-1-AP 1:500) with Raji cells lysate 1600 ug.

KCNQ5 Polyclonal antibody

KCNQ5 Polyclonal Antibody for WB, IP, ELISA
Cat No. 28000-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, ELISA

别名

Voltage-gated potassium channel subunit Kv7.5, Potassium voltage-gated channel subfamily KQT member 5, Kv7.5, KQT-like 5, KQT like 5

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inCalu-1 cells, HEL cells, Raji cells, mouse brain tissue, rat brain tissue
Positive IP detected inRaji cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28000-1-AP targets KCNQ5 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag27622

Product name: Recombinant human KCNQ5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 785-932 aa of NM_001160130

Sequence: MVASKENVQVAQSNLTKDRSMRKSFDMGGETLLSVCPMVPKDLGKSLSVQNLIRSTEELNIQLSGSESSGSRGSQDFYPKWRESKLFITDEEVGPEETETDTFDAAPQPAREAAFASDSLRTGRSRSSQSICKAGESTDALSLPHVKLK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 potassium voltage-gated channel, KQT-like subfamily, member 5
别名 Voltage-gated potassium channel subunit Kv7.5, Potassium voltage-gated channel subfamily KQT member 5, Kv7.5, KQT-like 5, KQT like 5
计算分子量 102 kDa
观测分子量100 kDa
GenBank蛋白编号NM_001160130
基因名称 KCNQ5
Gene ID (NCBI) 56479
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NR82
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Potassium voltage-gated channel subfamily Q member 5 (KCNQ5, also known as Kv7.5 and MRD46) is widely expressed in the brain and generates M-type current. It has a role in the regulation of neuronal excitability (PMID: 10816588). KCNQ5 mutations are associated with increased excitability and decreased repolarization reserve, lead to pathophysiology (PMID: 28669405).

实验方案

Product Specific Protocols
IP protocol for KCNQ5 antibody 28000-1-APDownload protocol
WB protocol for KCNQ5 antibody 28000-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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