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验证数据展示

  • WB analysis using 28000-1-AP

    WB analysis using 28000-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28000-1-AP (KCNQ5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IP experiment of Raji using 28000-1-AP

    IP experiment of Raji using 28000-1-AP

    IP result of anti-KCNQ5 (IP:28000-1-AP, 4ug; Detection:28000-1-AP 1:500) with Raji cells lysate 1600 ug.

KCNQ5 Polyclonal antibody

KCNQ5 Polyclonal Antibody for WB, IP, ELISA
Cat No. 28000-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, ELISA

别名

Voltage-gated potassium channel subunit Kv7.5, Potassium voltage-gated channel subfamily KQT member 5, Kv7.5, KQT-like 5, KQT like 5

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inCalu-1 cells, HEL cells, Raji cells, mouse brain tissue, rat brain tissue
Positive IP detected inRaji cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28000-1-AP targets KCNQ5 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 KCNQ5 fusion protein Ag27622 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 potassium voltage-gated channel, KQT-like subfamily, member 5
别名 Voltage-gated potassium channel subunit Kv7.5, Potassium voltage-gated channel subfamily KQT member 5, Kv7.5, KQT-like 5, KQT like 5
计算分子量 102 kDa
观测分子量100 kDa
GenBank蛋白编号NM_001160130
基因名称 KCNQ5
Gene ID (NCBI) 56479
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NR82
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Potassium voltage-gated channel subfamily Q member 5 (KCNQ5, also known as Kv7.5 and MRD46) is widely expressed in the brain and generates M-type current. It has a role in the regulation of neuronal excitability (PMID: 10816588). KCNQ5 mutations are associated with increased excitability and decreased repolarization reserve, lead to pathophysiology (PMID: 28669405).

实验方案

Product Specific Protocols
WB protocol for KCNQ5 antibody 28000-1-APDownload protocol
IP protocol for KCNQ5 antibody 28000-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols