验证数据展示
经过测试的应用
| Positive WB detected in | pig brain tissue, rat brain tissue, mouse brain tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U2OS cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:300-1:1200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
| IF | See 1 publications below |
产品信息
66774-1-Ig targets KCNQ2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat, pig |
| 文献引用反应性 | mouse, rat |
| 免疫原 |
CatNo: Ag25781 Product name: Recombinant human KCNQ2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-81 aa of BC000699 Sequence: MVQKSRNGGVYPGPSGEKKLKVGFVGLDPGAPDSTRDGALLIAGSEAPKRGSILSKPRAGGAGAGKPPKRNAFYRKLQNFL 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | potassium voltage-gated channel, KQT-like subfamily, member 2 |
| 别名 | 1D4A1, BFNC, EBN, EBN1, ENB1 |
| 计算分子量 | 96 kDa |
| 观测分子量 | 90 kDa |
| GenBank蛋白编号 | BC000699 |
| 基因名称 | KCNQ2 |
| Gene ID (NCBI) | 3785 |
| RRID | AB_2882120 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | O43526 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for KCNQ2 antibody 66774-1-Ig | Download protocol |
| IHC protocol for KCNQ2 antibody 66774-1-Ig | Download protocol |
| WB protocol for KCNQ2 antibody 66774-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Sci Rep Mir324 knockout regulates the structure of dendritic spines and impairs hippocampal long-term potentiation | ||
Hippocampus Impacted spike frequency adaptation associated with reduction of KCNQ2/3 exacerbates seizure activity in temporal lobe epilepsy | ||
Clin Exp Hypertens Effect of allisartan on blood pressure and left ventricular hypertrophy through Kv1.5 channels in hypertensive rats. |
