验证数据展示
经过测试的应用
| Positive WB detected in | fetal human brain tissue, human brain tissue |
| Positive IHC detected in | human brain tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
产品信息
60122-1-Ig targets KCNMB4 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag5618 Product name: Recombinant human KCNMB4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 46-176 aa of BC042446 Sequence: DLQATEANCTVLSVQQIGEVFECTFTCGADCRGTSQYPCVQVYVNNSESNSRALLHSDEHQLLTNPKCSYIPPCKRENQKNLESVMNWQQYWKDEIGSQPFTCYFNQHQRPDDVLLHRTHDEIVLLHCFLW 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | potassium large conductance calcium-activated channel, subfamily M, beta member 4 |
| 别名 | 4H7G3, BK channel subunit beta 4, BK channel subunit beta-4, BKbeta4, Calcium-activated potassium channel subunit beta-4 |
| 计算分子量 | 24 kDa |
| 观测分子量 | 25 kDa |
| GenBank蛋白编号 | BC042446 |
| 基因名称 | KCNMB4 |
| Gene ID (NCBI) | 27345 |
| RRID | AB_2280832 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q86W47 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for KCNMB4 antibody 60122-1-Ig | Download protocol |
| WB protocol for KCNMB4 antibody 60122-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Biomed Pharmacother Cytoprotective effects of the flavonoid quercetin by activating mitochondrial BKCa channels in endothelial cells. | ||
Mitochondrion Deficiency of the BKCa potassium channel displayed significant implications for the physiology of the human bronchial epithelium | ||
Peptides Apelin/APJ increased renal blood flow through endothelial BKCa channel induced p-eNOS and ET-1 in diabetic conditions |
