验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HepG2 cells, L02 cells, PC-3 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
14136-1-AP targets KCNK5 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag5355 Product name: Recombinant human KCNK5 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 244-499 aa of BC060793 Sequence: WKVSMFVEVHKAIKKRRRRRKESFESSPHSRKALQVKGSTASKDVNIFSFLSKKEETYNDLIKQIGKKAMKTSGGGETGPGPGLGPQGGGLPALPPSLVPLVVYSKNRVPTLEEVSQTLRSKGHVSRSPDEEAVARAPEDSSPAPEVFMNQLDRISEECEPWDAQDYHPLIFQDASITFVNTEAGLSDEETSKSSLEDNLAGEESPQQGAEAKAPLNMGEFPSSSESTFTSTESELSVPYEQLMNEYNKANSPKGT 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | potassium channel, subfamily K, member 5 |
| 别名 | Acid-sensitive potassium channel protein TASK-2, K2p5.1, TASK 2, TASK2, TWIK-related acid-sensitive K(+) channel 2 |
| 计算分子量 | 499 aa, 55 kDa |
| 观测分子量 | 70 kDa, 55 kDa |
| GenBank蛋白编号 | BC060793 |
| 基因名称 | KCNK5 |
| Gene ID (NCBI) | 8645 |
| RRID | AB_2280811 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O95279 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Potassium channel subfamily K member 5 (KCNK5, also termed TASK2) is a potassium channel mediating voltage-dependent outward-rectifying currents in response to membrane depolarization. Its voltage sensing is coupled to the potassium electrochemical gradient via an "ion flux gating" mechanism, in which gate opening is triggered by outward rather than inward ion movement (PMID: 26919430; 36063992; 9812978). Immunoblotting analysis of KCNK5 detects two protein bands at 55 kDa and 70 kDa, representing the monomeric and glycosylated forms of the KCNK5 potassium channel, respectively (PMID: 24285684; 26140335).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for KCNK5 antibody 14136-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
