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验证数据展示

  • WB analysis of mouse lung using 14135-1-AP

    WB analysis of mouse lung using 14135-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 14135-1-AP (Kir2.2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • FC experiment of HEK-293 using 14135-1-AP

    FC experiment of HEK-293 using 14135-1-AP

    1x10^6 HEK-293 cells were intracellularly stained with 0.5 ug Kir2.2 Polyclonal antibody (14135-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.5 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Kir2.2 Polyclonal antibody

Kir2.2 Polyclonal Antibody for WB, FC (Intra), ELISA
Cat No. 14135-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, FC (Intra), ELISA

别名

KCNJ12, IRK2, IRK 2, hkir2.2x, hIRK1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse lung tissue, rat lung tissue
Positive FC (Intra) detected inHEK-293 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2400
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14135-1-AP targets Kir2.2 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Kir2.2 fusion protein Ag5349 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 potassium inwardly-rectifying channel, subfamily J, member 12
别名 KCNJ12, IRK2, IRK 2, hkir2.2x, hIRK1
计算分子量 433 aa, 49 kDa
观测分子量 45-50 kDa
GenBank蛋白编号BC027982
基因名称 Kir2.2
Gene ID (NCBI) 3768
RRIDAB_2130478
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ14500
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Kir2.2 antibody 14135-1-APDownload protocol
FC protocol for Kir2.2 antibody 14135-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols