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验证数据展示

  • WB analysis of mouse cerebellum using 66931-1-Ig

    WB analysis of mouse cerebellum using 66931-1-Ig

    mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 66931-1-Ig (Kir4.1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Kir4.1 Monoclonal antibody

Kir4.1 Monoclonal Antibody for WB, ELISA
Cat No. 66931-1-Ig

产品说明书

CloneNo. 2D6A6
引用文献(2)

宿主/亚型

Mouse / IgG1

种属反应性

Human, Mouse and More (1)

应用

WB, ELISA and More (1)

别名

BIRK 10, KCNJ10, KCNJ13 PEN, KIR1.2, Kir4.1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse cerebellum tissue, mouse brain tissue, SH-SY5Y cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66931-1-Ig targets Kir4.1 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse samples.

经测试应用 WB, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 Human, Mouse
文献引用反应性mouse
免疫原

CatNo: Ag16785

Product name: Recombinant human KCNJ10 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 224-426 aa of BC034036

Sequence: ETIRFSQHAVVASHNGKPCLMIRVANMRKSLLIGCQVTGKLLQTHQTKEGENIRLNQVNVTFQVDTASDSPFLILPLTFYHVVDETSPLKDLPLRSGEGDFELVLILSGTVESTSATCQVRTSYLPEEILWGYEFTPAISLSASGKYIADFSLFDQVVKVASPSGLRDSTVRYGDPEKLKLEESLREQAEKEGSALSVRISNV

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 potassium inwardly-rectifying channel, subfamily J, member 10
别名 BIRK 10, KCNJ10, KCNJ13 PEN, KIR1.2, Kir4.1
计算分子量 379 aa, 41 kDa
观测分子量 40 kDa
GenBank蛋白编号BC034036
基因名称 Kir4.1
Gene ID (NCBI) 3766
RRIDAB_2882257
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP78508
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Kir4.1 antibody 66931-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIHC

J Ovarian Res

Ovarian tissue transplantation ameliorates osteoporosis and dyslipidaemia in ovariectomised mice

Authors - Encheng Zhou
View Article
mouseWB

Biochim Biophys Acta Mol Basis Dis

Involvement of Kir4.1 in pain insensitivity of the BTBR mouse model of autism spectrum disorder

Authors - Xiang Li
View Article