验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, human brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | SH-SY5Y cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12032-1-AP targets KCNIP3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag2665 Product name: Recombinant human KCNIP3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-256 aa of BC012850 Sequence: MQPAKEVTKASDGSLLGDLGHTPLSKKEGIKWQRPRLSRQALMRCCLVKWILSSTAPQGSDSSDSELELSTVRHQPEGLDQLQAQTKFTKKELQSLYRGFKNECPTGLVDEDTFKLIYAQFFPQGDATTYAHFLFNAFDADGNGAIHFEDFVVGLSILLRGTVHEKLKWAFNLYDINKDGYITKEEMLAIMKSIYDMMGRHTYPILREDAPAEHVERFFEKMDRNQDGVVTIEEFLEACQKDENIMSSMQLFENVI 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | Kv channel interacting protein 3, calsenilin |
| 别名 | A-type potassium channel modulatory protein 3, Calsenilin, DRE-antagonist modulator, Kv channel-interacting protein 3 |
| 计算分子量 | 256 aa, 29 kDa |
| 观测分子量 | 29 kDa, 27 kDa |
| GenBank蛋白编号 | BC012850 |
| 基因名称 | KCNIP3 |
| Gene ID (NCBI) | 30818 |
| RRID | AB_2234348 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9Y2W7 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
KCNIP3 also known as Calsenilin, CSEN, DREAM, or KCHIP3, is a protein-coding gene located on human chromosome 2q11.1 . It encodes a small calcium-binding protein belonging to the recoverin branch of the EF-hand superfamily. KCNIP3 is an integral subunit of native Kv4 channel complexes, regulating A-type potassium currents in response to intracellular calcium changes, thereby modulating neuronal excitability. KCNIP3 interacts with presenilin 1 (PSEN1) and presenilin 2 (PSEN2), influencing γ-secretase activity and β-amyloid formation, potentially linking it to Alzheimer's disease pathology.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for KCNIP3 antibody 12032-1-AP | Download protocol |
| IHC protocol for KCNIP3 antibody 12032-1-AP | Download protocol |
| IP protocol for KCNIP3 antibody 12032-1-AP | Download protocol |
| WB protocol for KCNIP3 antibody 12032-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
