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验证数据展示

  • WB analysis of human cerebellum using 14212-1-AP

    WB analysis of human cerebellum using 14212-1-AP

    human cerebellum tissue were subjected to SDS PAGE followed by western blot with 14212-1-AP (KChIP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas cancer using 14212-1-AP

    IHC staining of human pancreas cancer using 14212-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 14212-1-AP (KChIP1 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human pancreas cancer using 14212-1-AP

    IHC staining of human pancreas cancer using 14212-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 14212-1-AP (KChIP1 antibody) at dilution of 1:100 (under 40x lens).

  • IP experiment of fetal human brain using 14212-1-AP

    IP experiment of fetal human brain using 14212-1-AP

    IP result of anti-KChIP1 (IP:14212-1-AP, 4ug; Detection:14212-1-AP 1:300) with fetal human brain tissue lysate 3500 ug.

  • FC experiment of HepG2 using 14212-1-AP

    FC experiment of HepG2 using 14212-1-AP

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug KChIP1 Polyclonal antibody (14212-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

KChIP1 Polyclonal antibody

KChIP1 Polyclonal Antibody for WB, IHC, FC (Intra), IP, ELISA
Cat No. 14212-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, FC (Intra), IP, ELISA

别名

KCNIP1, A-type potassium channel modulatory protein 1, Kv channel-interacting protein 1, Potassium channel-interacting protein 1, VABP

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inhuman cerebellum tissue, human brain tissue
Positive IP detected infetal human brain tissue
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14212-1-AP targets KChIP1 in WB, IHC, FC (Intra), IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, FC (Intra), IP, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag5432

Product name: Recombinant human KCNIP1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-216 aa of BC050375

Sequence: MGAVMGTFSSLQTKQRRPSKDKIEDELEMTMVCHRPEGLEQLEAQTNFTKRELQVLYRGFKNECPSGVVNEDTFKQIYAQFFPHGDASTYAHYLFNAFDTTQTGSVKFEDFVTALSILLRGTVHEKLRWTFNLYDINKDGYINKEEMMDIVKAIYDMMGKYTYPVLKEDTPRQHVDVFFQKMDKNKDGIVTLDEFLESCQEDDNIMRSLQLFQNVM

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Kv channel interacting protein 1
别名 KCNIP1, A-type potassium channel modulatory protein 1, Kv channel-interacting protein 1, Potassium channel-interacting protein 1, VABP
计算分子量 227 aa, 27 kDa
观测分子量 25 kDa
GenBank蛋白编号BC050375
基因名称 KCNIP1
Gene ID (NCBI) 30820
RRIDAB_2265412
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NZI2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Human K(v) channel interacting protein 1 (KCHIP1) is a new member of the neural calcium binding protein superfamily. Members of the KCNIP family are small calcium binding proteins. They all have EF-hand-like domains, and differ from each other in the N-terminus. They are integral subunit components of native Kv4 channel complexes. They may regulate A-type currents, and hence neuronal excitability, in response to changes in intracellular calcium. KChIP1 is a neuronal calcium sensor protein that is predominantly expressed at GABAergic synapses and it has been related with modulation of K(+) channels, GABAergic transmission and cell death.

实验方案

Product Specific Protocols
WB protocol for KChIP1 antibody 14212-1-APDownload protocol
IHC protocol for KChIP1 antibody 14212-1-APDownload protocol
IP protocol for KChIP1 antibody 14212-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols