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KChIP1 Polyclonal antibody

KChIP1 Polyclonal Antibody for FC, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC, IHC, IP, WB,ELISA

Conjugate

Unconjugated

Cat No : 14212-1-AP

Print datasheet

Synonyms

KCHIP1, KCNIP1, VABP, Vesicle APC binding protein

验证数据展示

  • WB analysis of human cerebellum using 14212-1-AP

    WB analysis of human cerebellum using 14212-1-AP

    human cerebellum tissue were subjected to SDS PAGE followed by western blot with 14212-1-AP (KChIP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas cancer using 14212-1-AP

    IHC staining of human pancreas cancer using 14212-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 14212-1-AP (KChIP1 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human pancreas cancer using 14212-1-AP

    IHC staining of human pancreas cancer using 14212-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 14212-1-AP (KChIP1 antibody) at dilution of 1:100 (under 40x lens).

  • IP experiment of fetal human brain using 14212-1-AP

    IP experiment of fetal human brain using 14212-1-AP

    IP result of anti-KChIP1 (IP:14212-1-AP, 4ug; Detection:14212-1-AP 1:300) with fetal human brain tissue lysate 3500 ug.

  • FC experiment of HepG2 using 14212-1-AP

    FC experiment of HepG2 using 14212-1-AP

    1X10^6 HepG2 cells were stained with 0.2ug KChIP1 antibody (14212-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

经过测试的应用

Positive WB detected inhuman cerebellum tissue, human brain tissue
Positive IP detected infetal human brain tissue
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14212-1-AP targets KChIP1 in WB, IP, FC, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications FC, IHC, IP, WB,ELISA
Tested Reactivity human
Immunogen KChIP1 fusion protein Ag5432 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Kv channel interacting protein 1
Synonyms KCHIP1, KCNIP1, VABP, Vesicle APC binding protein
Calculated Molecular Weight 227 aa, 27 kDa
Observed Molecular Weight 25 kDa
GenBank Accession NumberBC050375
Gene Symbol KCNIP1
Gene ID (NCBI) 30820
RRIDAB_2265412
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NZI2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Human K(v) channel interacting protein 1 (KCHIP1) is a new member of the neural calcium binding protein superfamily. Members of the KCNIP family are small calcium binding proteins. They all have EF-hand-like domains, and differ from each other in the N-terminus. They are integral subunit components of native Kv4 channel complexes. They may regulate A-type currents, and hence neuronal excitability, in response to changes in intracellular calcium. KChIP1 is a neuronal calcium sensor protein that is predominantly expressed at GABAergic synapses and it has been related with modulation of K(+) channels, GABAergic transmission and cell death.

实验方案

Product Specific Protocols
WB protocol for KChIP1 antibody 14212-1-APDownload protocol
IHC protocol for KChIP1 antibody 14212-1-APDownload protocol
IP protocol for KChIP1 antibody 14212-1-APDownload protocol
FC protocol for KChIP1 antibody 14212-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols