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验证数据展示

  • WB analysis using 83674-1-RR

    WB analysis using 83674-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83674-1-RR (KCNH7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of pig brain using 83674-1-RR

    WB analysis of pig brain using 83674-1-RR

    pig brain tissue were subjected to SDS PAGE followed by western blot with 83674-1-RR (KCNH7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 83674-1-RR

    IHC staining of mouse brain using 83674-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83674-1-RR (KCNH7 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83674-1-RR

    Affinity and Kinetic Characterization of 83674-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83674-1-RR against Human KCNH7 were performed. The affinity constant is below 1 pM.

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KCNH7 Recombinant monoclonal antibody

KCNH7 Uni-rAb® Recombinant Antibody for WB, IHC, ELISA
Cat No. 83674-1-RR

产品说明书

CloneNo. 240644B5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, pig

应用

WB, IHC, ELISA

别名

ERG-3, ERG3, ERG 3, Eag-related protein 3, Eag related protein 3

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse brain tissue, pig brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83674-1-RR targets KCNH7 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原

CatNo: Ag4532

Product name: Recombinant human KCNH7 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-297 aa of BC035815

Sequence: MPVRRGHVAPQNTFLGTIIRKFEGQNKKFIIANARVQNCAIIYCNDGFCEMTGFSRPDVMQKPCTCDFLHGPETKRHDIAQIAQALLGSEERKVEVTYYHKNGSTFICNTHIIPVKNQEGVAMMFIINFEYVTDNENAATPERVNPILPIKTVNRKFFGFKFPGLRVLTYRKQSLPQEDPDVVVIDSSKHSDDSVAMKHFKSPTKESCSPSEADDTKALIQPSKCSPLVNISGPLDHSSPKRQWDRLYPDMLQSSSQLSHSRSRESLCSIRRASSVHDIEGFGVHPKNIFRDRHASE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 potassium voltage-gated channel, subfamily H (eag-related), member 7
别名 ERG-3, ERG3, ERG 3, Eag-related protein 3, Eag related protein 3
计算分子量 1196 aa, 135 kDa
观测分子量 135 kDa
GenBank蛋白编号BC035815
基因名称 KCNH7
Gene ID (NCBI) 90134
RRIDAB_3671279
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9NS40
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Potassium voltage-gated channel subfamily H member 7 is a protein that in humans is encoded by the KCNH7 gene. The protein encoded by this gene is a voltage-gated potassium channel subunit. KCNH7 is pore-forming (alpha) subunit of voltage-gated potassium channel. Channel properties may be modulated by cAMP and subunit assembly.

实验方案

Product Specific Protocols
WB protocol for KCNH7 antibody 83674-1-RRDownload protocol
IHC protocol for KCNH7 antibody 83674-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols