验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293T cells, MDA-MB-231 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
30853-1-AP targets KCNH2 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag34230 Product name: Recombinant human KCNH2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 880-1159 aa of NM_000238 Sequence: GFSRQRKRKLSFRRRTDKDTEQPGEVSALGPGRAGAGPSSRGRPGGPWGESPSSGPSSPESSEDEGPGRSSSPLRLVPFSSPRPPGEPPGGEPLMEDCEKSSDTCNPLSGAFSGVSNIFSFWGDSRGRQYQELPRCPAPTPSLLNIPLSSPGRRPRGDVESRLDALQRQLNRLETRLSADMATVLQLLQRQMTLVPPAYSAVTTPGPGPTSTSPLLPVSPLPTLTLDSLSQVSQFMACEELPPGAPELPQEGPTRRLSLPGQLGALTSQPLHRHGSDPGS 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | potassium voltage-gated channel, subfamily H (eag-related), member 2 |
| 别名 | Eag homolog, Eag related protein 1, Eag-related protein 1, ERG, ERG 1 |
| 计算分子量 | 127 kDa |
| 观测分子量 | 100-120 kDa |
| GenBank蛋白编号 | NM_000238 |
| 基因名称 | KCNH2 |
| Gene ID (NCBI) | 3757 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q12809 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Voltage-gated inwardly rectifying potassium channel KCNH2 (also known as ERG1, Kv11.1, and HERG) is a key member of the voltage-gated potassium (Kv) channel family (PMID: 16382104). Growing evidence has indicated that the association between KCNH2 and hypoglycemia, an adverse effect induced by KCNH2 blockers, implies a potential regulatory role of this channel in the maintenance of blood glucose homeostasis (PMID: 21224236; 31863282). Mutations in the KCNH2 gene are recognized as one of the etiological factors of congenital long QT syndrome (LQTS), a rare syndrome that carries an increased risk of cardiac arrhythmias, including the polymorphic ventricular tachycardia termed torsades de pointes (TdP) (PMID: 17143043; 16554806).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for KCNH2 antibody 30853-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
