• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 26426-1-AP

    WB analysis using 26426-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26426-1-AP (KCNH1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IP experiment of rat brain using 26426-1-AP

    IP experiment of rat brain using 26426-1-AP

    IP result of anti-KCNH1 (IP:26426-1-AP, 4ug; Detection:26426-1-AP 1:5000) with rat brain tissue lysate 1720 ug.

  • IP experiment of mouse brain using 26426-1-AP

    IP experiment of mouse brain using 26426-1-AP

    IP result of anti-KCNH1 (IP:26426-1-AP, 4ug; Detection:26426-1-AP 1:5000) with mouse brain tissue lysate 2120 ug.

  • IHC staining of mouse brain using 26426-1-AP

    IHC staining of mouse brain using 26426-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26426-1-AP (KCNH1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat brain using 26426-1-AP

    IHC staining of rat brain using 26426-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 26426-1-AP (KCNH1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat brain using 26426-1-AP

    IHC staining of rat brain using 26426-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 26426-1-AP (KCNH1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

KCNH1 Polyclonal antibody

KCNH1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 26426-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

EAG, EAG channel 1, EAG1, Ether-a-go-go potassium channel 1, h eag

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inrat brain tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26426-1-AP targets KCNH1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 KCNH1 fusion protein Ag16647 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 potassium voltage-gated channel, subfamily H (eag-related), member 1
别名 EAG, EAG channel 1, EAG1, Ether-a-go-go potassium channel 1, h eag
计算分子量 989 aa, 111 kDa
观测分子量111 kDa
GenBank蛋白编号BC113709
基因名称 KCNH1
Gene ID (NCBI) 3756
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO95259
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Potassium voltage-gated channel subfamily H member 1 (KCNH1, also known as Kv10.1 and EAG) a member of the EAG (ether-à-go-go) family, which is involved in intracellular signaling, cell proliferation, and tumorigenesis (PMID: 35639255). KCNH1 is mainly expressed in the adult central nervous system. It also plays a role in controlling K+ fux that regulates resting membrane potential in excitable and non-excitable cells and is activated at the onset of myoblast differentiation (PMID: 29333676; 27029528; 9738473).

实验方案

Product Specific Protocols
WB protocol for KCNH1 antibody 26426-1-APDownload protocol
IHC protocol for KCNH1 antibody 26426-1-APDownload protocol
IP protocol for KCNH1 antibody 26426-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols