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验证数据展示

  • WB analysis using 85133-3-RR (same clone as 85133-3-PBS)

    WB analysis using 85133-3-RR (same clone as 85133-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85133-3-RR (KCND2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85133-3-PBS in a different storage buffer formulation.

  • WB analysis of DU 145 using 85133-3-RR (same clone as 85133-3-PBS)

    WB analysis of DU 145 using 85133-3-RR (same clone as 85133-3-PBS)

    DU 145 cells were subjected to SDS PAGE followed by western blot with 85133-3-RR (KCND2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85133-3-PBS in a different storage buffer formulation.

  • IHC staining of mouse cerebellum using 85133-3-RR (same clone as 85133-3-PBS)

    IHC staining of mouse cerebellum using 85133-3-RR (same clone as 85133-3-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 85133-3-RR (Kv4.2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85133-3-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 85133-3-RR (same clone as 85133-3-PBS)

    IF Staining of mouse brain using 85133-3-RR (same clone as 85133-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using Kv4.2 antibody (85133-3-RR, Clone: 242754F4 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85133-3-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01834-2

    Cytometric bead array standard curve of MP01834-2

    Cytometric bead array standard curve of MP01834-2, Kv4.2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85133-4-PBS. Detection antibody: 85133-3-PBS. Standard: Ag15879. Range: 1.563-100 ng/mL.

  • Affinity and Kinetic Characterization of 85133-3-RR

    Affinity and Kinetic Characterization of 85133-3-RR

    Biolayer interferometry (BLl) kinetic assays of 85133-3-RR against Human Kv4.2 were performed. The affinity constant is below 1 pM.

Kv4.2 Recombinant antibody, PBS Only (Detector)

Kv4.2 Uni-rAbTM Recombinant Antibody for WB, IHC, IF-P, Cytometric bead array, Indirect ELISA
Cat No. 85133-3-PBS

产品说明书

CloneNo. 242754F4

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

别名

KCND2, 242754F4, KIAA1044, Potassium voltage-gated channel subfamily D member 2, RK5

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

85133-3-PBS targets Kv4.2 as part of a matched antibody pair:

MP01834-2: 85133-4-PBS capture and 85133-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF-P, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 Kv4.2 fusion protein Ag15879 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 potassium voltage-gated channel, Shal-related subfamily, member 2
别名 KCND2, 242754F4, KIAA1044, Potassium voltage-gated channel subfamily D member 2, RK5
计算分子量 630 aa, 71 kDa
观测分子量75 kDa
GenBank蛋白编号BC110449
基因名称 KCND2
Gene ID (NCBI) 3751
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9NZV8
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Voltage-gated potassium or Kv channels, specifically those mediating low threshold, rapidly inactivating Ito and IA currents, are known to regulate cardiac and neuronal membrane excitability, respectively (PMID: 12829703). Voltage-gated potassium channel subunit Kv4.2, encoded by the KCND2 gene, belongs to the potassium channel family and D (Shal) subfamily. It is a pore-forming alpha subunit of voltage-gated rapidly inactivating A-type potassium channels. Kv4.2 is highly expressed in the brain (PMID: 10551270). It is a major constituent of A-type potassium currents and a key regulator of neuronal membrane excitability (PMID: 22539834).