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验证数据展示

  • WB analysis using 26590-1-AP

    WB analysis using 26590-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26590-1-AP (KCC4/SLC12A7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 26590-1-AP

    IF Staining of A431 using 26590-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using KCC4/SLC12A7 antibody (26590-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

KCC4/SLC12A7 Polyclonal antibody

KCC4/SLC12A7 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 26590-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

K Cl cotransporter 4, KCC4, KCC4/SLC12A7, SLC12A7

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inBGC-823 cells, NCI-H1299 cells, Raji cells, SH-SY5Y cells
Positive IF/ICC detected inA431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26590-1-AP targets KCC4/SLC12A7 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 KCC4/SLC12A7 fusion protein Ag24954 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 solute carrier family 12 (potassium/chloride transporters), member 7
别名 K Cl cotransporter 4, KCC4, KCC4/SLC12A7, SLC12A7
观测分子量119 kDa
GenBank蛋白编号BC098390
基因名称 KCC4/SLC12A7
Gene ID (NCBI) 10723
RRIDAB_2918103
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y666
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KCC4, also named as Solute carrier family 12 member 7(SLC12A7), is a 1083 amino-acid protein, which belongs to the SLC12A transporter family. As a multi-pass membrane protein, the molecular weight of KCC4 is 119 kDa. KCC4 mediates electroneutral potassium-chloride cotransport when activated by cell swelling. Moreover, KCC4 may mediate K+ uptake into Deiters' cells in the cochlea and contribute to K+ recycling in the inner ear. KCC4 is important for the survival of cochlear outer and inner hair cells and the maintenance of the organ of Corti(Uniprot, GeneID:10723).

实验方案

Product Specific Protocols
WB protocol for KCC4/SLC12A7 antibody 26590-1-APDownload protocol
IF protocol for KCC4/SLC12A7 antibody 26590-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols