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KCC4/SLC12A7 Polyclonal antibody

KCC4/SLC12A7 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat No : 26590-1-AP

Print datasheet

Synonyms

K Cl cotransporter 4, KCC4, KCC4/SLC12A7, SLC12A7

验证数据展示

  • WB analysis using 26590-1-AP

    WB analysis using 26590-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26590-1-AP (KCC4/SLC12A7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 26590-1-AP

    IF Staining of A431 using 26590-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using KCC4/SLC12A7 antibody (26590-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inBGC-823 cells, NCI-H1299 cells, Raji cells, SH-SY5Y cells
Positive IF detected inA431 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26590-1-AP targets KCC4/SLC12A7 in WB, IF, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, ELISA
Tested Reactivity human
Immunogen KCC4/SLC12A7 fusion protein Ag24954 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name solute carrier family 12 (potassium/chloride transporters), member 7
Synonyms K Cl cotransporter 4, KCC4, KCC4/SLC12A7, SLC12A7
Observed Molecular Weight119 kDa
GenBank Accession NumberBC098390
Gene Symbol SLC12A7
Gene ID (NCBI) 10723
RRIDAB_2918103
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y666
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KCC4, also named as Solute carrier family 12 member 7(SLC12A7), is a 1083 amino-acid protein, which belongs to the SLC12A transporter family. As a multi-pass membrane protein, the molecular weight of KCC4 is 119 kDa. KCC4 mediates electroneutral potassium-chloride cotransport when activated by cell swelling. Moreover, KCC4 may mediate K+ uptake into Deiters' cells in the cochlea and contribute to K+ recycling in the inner ear. KCC4 is important for the survival of cochlear outer and inner hair cells and the maintenance of the organ of Corti(Uniprot, GeneID:10723).

实验方案

Product Specific Protocols
WB protocol for KCC4/SLC12A7 antibody 26590-1-APDownload protocol
IF protocol for KCC4/SLC12A7 antibody 26590-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols