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验证数据展示

  • WB analysis using 25577-1-AP

    WB analysis using 25577-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25577-1-AP (KBTBD10 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human heart using 25577-1-AP

    IHC staining of human heart using 25577-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 25577-1-AP (KBTBD10 Antibody) at dilution of 1:100 (under 40x lens).

  • IHC staining of human skeletal muscle using 25577-1-AP

    IHC staining of human skeletal muscle using 25577-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 25577-1-AP (KBTBD10 Antibody) at dilution of 1:50 (under 40x lens).

KBTBD10 Polyclonal antibody

KBTBD10 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 25577-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

KBTBD10, Kel like protein 23, Kelch related protein 1, Kelch-like protein 41, KLH41, KLHL41, KRP1, SARCOSIN

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse heart tissue, mouse skeletal muscle tissue
Positive IHC detected inhuman heart tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25577-1-AP targets KBTBD10 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 KBTBD10 fusion protein Ag22311 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 kelch repeat and BTB (POZ) domain containing 10
别名 KBTBD10, Kel like protein 23, Kelch related protein 1, Kelch-like protein 41, KLH41, KLHL41, KRP1, SARCOSIN
计算分子量 606 aa, 68 kDa
观测分子量 68 kDa
GenBank蛋白编号BC006534
基因名称 KBTBD10
Gene ID (NCBI) 10324
RRIDAB_2880137
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO60662
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KBTBD10, also named as KBTBD10 or KRP1, is a 606 amino acid protein, which contains one BACK (BTB/Kelch associated) domain, one BTB (POZ) domain and five Kelch repeats. KBTBD10 localizes in the cytoplasm and is expressed in Sarcomeric muscle. KBTBD10 is involved in skeletal muscle development and differentiation. KBTBD10 regulates proliferation and differentiation of myoblasts and plays a role in myofibril assembly by promoting lateral fusion of adjacent thin fibrils into mature, wide myofibrils.

实验方案

Product Specific Protocols
WB protocol for KBTBD10 antibody 25577-1-APDownload protocol
IHC protocol for KBTBD10 antibody 25577-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols