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验证数据展示

  • WB analysis using 14951-1-AP (same clone as 14951-1-PBS)

    WB analysis using 14951-1-AP (same clone as 14951-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14951-1-AP (KARS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14951-1-PBS in a different storage buffer formulation.

  • IHC staining of human brown disease using 14951-1-AP (same clone as 14951-1-PBS)

    IHC staining of human brown disease using 14951-1-AP (same clone as 14951-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brown disease slide using 14951-1-AP (KARS antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14951-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with KARS antibody included
  • IHC staining of human brown disease using 14951-1-AP (same clone as 14951-1-PBS)

    IHC staining of human brown disease using 14951-1-AP (same clone as 14951-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brown disease slide using 14951-1-AP (KARS antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14951-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with KARS antibody included
  • IF Staining of A431 using 14951-1-AP (same clone as 14951-1-PBS)

    IF Staining of A431 using 14951-1-AP (same clone as 14951-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using KARS antibody (14951-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14951-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

KARS Polyclonal antibody, PBS Only

KARS Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 14951-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

Lysyl-tRNA synthetase, Lysine--tRNA ligase, KIAA0070, KARS1, EC:2.7.7.-

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy® KARS Ready-To-Use IHC Kit. KARS primary antibody included.

产品信息

14951-1-PBS targets KARS in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag6778

Product name: Recombinant human KARS protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 246-597 aa of BC004132

Sequence: IRSKIITYIRSFLDELGFLEIETPMMNIIPGGAVAKPFITYHNELDMNLYMRIAPELYHKMLVVGGIDRVYEIGRQFRNEGIDLTHNPEFTTCEFYMAYADYHDLMEITEKMVSGMVKHITGSYKVTYHPDGPEGQAYDVDFTPPFRRINMVEELEKALGMKLPETNLFETEETRKILDDICVAKAVECPPPRTTARLLDKLVGEFLEVTCINPTFICDHPQIMSPLAKWHRSKEGLTERFELFVMKKEICNAYTELNDPMRQRQLFEEQAKAKAAGDDEAMFIDENFCTALEYGLPPTAGWGMGIDRVAMFLTDSNNIKEVLLFPAMKPEDKKENVATTDTLESTTVGTSV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 lysyl-tRNA synthetase
别名 Lysyl-tRNA synthetase, Lysine--tRNA ligase, KIAA0070, KARS1, EC:2.7.7.-
计算分子量 68 kDa
观测分子量 68 kDa
GenBank蛋白编号BC004132
基因名称 KARS
Gene ID (NCBI) 3735
RRIDAB_2128158
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ15046
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

KARS (lysyl-tRNA synthetase) is a gene that encodes an enzyme critical for protein synthesis, specifically responsible for attaching the amino acid lysine to its corresponding transfer RNA (tRNA) in both the cytoplasm and mitochondria. This enzyme plays a crucial role in the process of aminoacylation, which is essential for accurate protein translation from mRNA sequences. Mutations in the KARS gene have been associated with a variety of neurological and systemic disorders, reflecting the enzyme's importance in cellular function.

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