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Host
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Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
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Compatible Species
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Conjugate
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验证数据展示

  • WB analysis using 85693-4-RR (same clone as 85693-4-PBS)

    WB analysis using 85693-4-RR (same clone as 85693-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85693-4-RR (KAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85693-4-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 85693-4-RR (same clone as 85693-4-PBS)

    IF Staining of HepG2 using 85693-4-RR (same clone as 85693-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using KAP1 antibody (85693-4-RR, Clone: 243043D10 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85693-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85693-4-RR

    Affinity and Kinetic Characterization of 85693-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85693-4-RR against Human KAP1 were performed. The affinity constant is 8.82 nM.

KAP1 Recombinant antibody, PBS Only

KAP1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 85693-4-PBS

产品说明书

CloneNo. 243043D10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Indirect ELISA

别名

TRIM28, E3 SUMO-protein ligase TRIM28, EC:2.3.2.27, KAP 1, KAP-1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy KAP1 Ready-To-Use IHC Kit. KAP1 primary antibody included.

产品信息

85693-4-PBS targets KAP1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原 KAP1 fusion protein Ag7350 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 tripartite motif-containing 28
别名 TRIM28, E3 SUMO-protein ligase TRIM28, EC:2.3.2.27, KAP 1, KAP-1
计算分子量 89 kDa
观测分子量100-120 kDa
GenBank蛋白编号BC004978
基因名称 KAP1
Gene ID (NCBI) 10155
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ13263
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

KAP1, also named as TRIM28 or RNF96, is a 835 amino acid protein, which contain one RING-type zinc finger, one PHD-type zinc finger, one bromo domain and two B box-type zinc fingers. KAP1 localizes in the nucleus and Belongs to the TRIM/RBCC family. KAP1 is a nuclear corepressor for KRAB domain-containing zinc finger proteins and mediates gene silencing by recruiting CHD3, a subunit of the nucleosome remodeling and deacetylation (NuRD) complex, and SETDB1 to the promoter regions of KRAB target genes. KAP1 is expressed in all tissues tested including spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes. The calcualted molecular weight of KAP1 is 89 kDa, but modified KAP1 is about 100 kDa. (PMID: 18590578 )