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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Compatible Species
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Conjugate
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验证数据展示

  • WB analysis using 27767-1-AP

    WB analysis using 27767-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27767-1-AP (Jagged1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Jagged1 Polyclonal antibody

Jagged1 Polyclonal Antibody for WB, ELISA
Cat No. 27767-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHUVEC cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27767-1-AP targets Jagged1 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 Jagged1 fusion protein Ag26633 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 jagged 1 (Alagille syndrome)
别名
计算分子量 1218 aa, 134 kDa
观测分子量150-180 kDa
GenBank蛋白编号BC098393
基因名称 Jagged1
Gene ID (NCBI) 182
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP78504
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Jagged1 is a type I, membrane- anchored, multidomain protein and a ligand of the Notch receptor. The receptor-ligand interaction can trigger the Notch signaling and activate transcription factors that play key roles in cell differentiation and morphogenesis. The predicted MW of Jagged1 is 134 kDa, while higher MW around 150-180 kDa had been observed in literations (PMID: 9716576, 9596653).

实验方案

Product Specific Protocols
WB protocol for Jagged1 antibody 27767-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols