验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, mouse brain tissue, rat brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
32471-1-AP targets JPH4 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag38425 Product name: Recombinant human JPH4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 450-600 aa of NM_001146028.1 Sequence: GLTPSEGSPELPSSPASSRQPWRPPACRSPLPPGGDQGPFSSPKAWPEEWGGAGAQAEELAGYEAEDEAGMQGPGPRDGSPLLGGCSDSSGSLREEEGEDEEPLPPLRAPAGTEPEPIAMLVLRGSSSRGPDAGCLTEELGEPAATERPAQ 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | junctophilin 4 |
| 别名 | KIAA1831, Junctophilin-like 1 protein, Junctophilin-4, JPHL1, JP-4 |
| 计算分子量 | 66kDa，628aa |
| 观测分子量 | 70 kDa |
| GenBank蛋白编号 | NM_001146028.1 |
| 基因名称 | JPH4 |
| Gene ID (NCBI) | 84502 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q96JJ6 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
JPH4 (Junctophilin 4) is a member of the junctophilin family of transmembrane proteins. JPH4 is involved in the formation of junctional membrane complexes between the plasma membrane and the endoplasmic/sarcoplasmic reticulum in excitable cells. It plays a crucial role in maintaining the structural integrity and function of these complexes. JPH4 is biased in expression in the brain (RPKM 34.3) and endometrium (RPKM 8.7), among other tissues.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for JPH4 antibody 32471-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
