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验证数据展示

  • WB analysis of HEK-293 using 25979-1-AP

    WB analysis of HEK-293 using 25979-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 25979-1-AP (JPH1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human skeletal muscle using 25979-1-AP

    IHC staining of human skeletal muscle using 25979-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 25979-1-AP (JPH1 antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human skeletal muscle using 25979-1-AP

    IHC staining of human skeletal muscle using 25979-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 25979-1-AP (JPH1 antibody) at dilution of 1:200 (under 40x lens).

  • WB analysis using 25979-1-AP

    WB analysis using 25979-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25979-1-AP (JPH1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

JPH1 Polyclonal antibody

JPH1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 25979-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

JP 1, JP1, JPH1, junctophilin 1, Junctophilin type 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, mouse heart tissue, rat heart tissue
Positive IHC detected inhuman skeletal muscle tissue, human skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25979-1-AP targets JPH1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag23270

Product name: Recombinant human JPH1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 464-639 aa of BC140875

Sequence: RSPEASPKHSHSPASSPKPLKKQNPSSGARLNQDKRSVADEQVTAIVNKPLMSKAPTKEAGAVVPQSKYSGRHHIPNPSNGELHSQYHGYYVKLNAPQHPPVDVEDGDGSSQSSSALVHKPSANKWSPSKSVTKPVAKESKAEPKAKKSELAIPKNPASNDSCPALEKEANSGPNS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 junctophilin 1
别名 JP 1, JP1, JPH1, junctophilin 1, Junctophilin type 1
计算分子量 72 kDa
观测分子量 72 kDa
GenBank蛋白编号BC140875
基因名称 JPH1
Gene ID (NCBI) 56704
RRIDAB_2880317
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9HDC5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Junctophilin-1 is a protein that in humans is encoded by the JPH1 gene. This gene is a member of the junctophilin gene family. Junctional complexes between the plasma membrane and endoplasmic/sarcoplasmic reticulum are a common feature of all excitable cell types and mediate cross talk between cell surface and intracellular ion channels. The protein encoded by this gene is a component of junctional complexes and is composed of a C-terminal hydrophobic segment spanning the endoplasmic/sarcoplasmic reticulum membrane and a remaining cytoplasmic domain that shows specific affinity for the plasma membrane.

实验方案

Product Specific Protocols
WB protocol for JPH1 antibody 25979-1-APDownload protocol
IHC protocol for JPH1 antibody 25979-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols