验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, Jurkat cells, MCF-7 cells |
Planning an IHC experiment? We recommend our IHCeasy JMJD5 Ready-To-Use IHC Kit. JMJD5 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
84804-4-RR targets JMJD5 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag15115 Product name: Recombinant human JMJD5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 67-416 aa of BC027911 Sequence: SSEVILDYSWEKLNTGTWQDVDKDWRRVYAIGCLLKALCLCQAPEDANTVAAALRVCDMGLLMGAAILGDILLKVAAILQTHLPGKRPARGSLPEQPCTKKARADHGLIPDVKLEKTVPRLHRPSLQHFREQFLVPGRPVILKGVADHWPCMQKWSLEYIQEIAGCRTVPVEVGSRYTDEEWSQTLMTVNEFISKYIVNEPRDVGYLAQHQLFDQIPELKQDISIPDYCSLGDGEEEEITINAWFGPQGTISPLHQDPQQNFLVQVMGRKYIRLYSPQESGALYPHDTHLLHNTSQVDVENPDLEKFPKFAKAPFLSCILSPGEILFIPVKYWHYVRALDLSFSVSFWWS 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | jumonji domain containing 5 |
| 别名 | 242097E10, 3R, Bifunctional peptidase and arginyl-hydroxylase JMJD5, EC:1.14.11.73, JmjC domain-containing protein 5 |
| 计算分子量 | 416 aa, 47 kDa |
| 观测分子量 | 50-60 kDa |
| GenBank蛋白编号 | BC027911 |
| 基因名称 | JMJD5 |
| Gene ID (NCBI) | 79831 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | Q8N371 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for JMJD5 antibody 84804-4-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
