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JMJD5 Polyclonal antibody

JMJD5 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat No : 20999-1-AP

Print datasheet

Synonyms

JMJD5, jumonji domain containing 5, KDM8, Lysine specific demethylase 8

验证数据展示

  • WB analysis using 20999-1-AP

    WB analysis using 20999-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20999-1-AP (JMJD5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHepG2 cells, Jurkat cells, MCF-7 cells, MDA-MB-453s cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20999-1-AP targets JMJD5 in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA
Tested Reactivity human
Immunogen JMJD5 fusion protein Ag15115 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name jumonji domain containing 5
Synonyms JMJD5, jumonji domain containing 5, KDM8, Lysine specific demethylase 8
Calculated Molecular Weight 416 aa, 47 kDa
Observed Molecular Weight50-60 kDa
GenBank Accession NumberBC027911
Gene Symbol JMJD5
Gene ID (NCBI) 79831
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N371
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

JmjC (Jumonji-C) domain-containing 5 (JMJD5) (also called KDM8) is a 2-oxoglutarate (2-OG) dependent oxygenase of the JmjC subfamily. By shuttling between the nucleus and cytoplasm, JMJD5 could either associate with histones or cytosolic proteins, and thus plays an important role in embryonic development (PMID:22241836;22402282), circadian regulation (PMID:21115819), osteoclastogenesis (PMID:22375008), and stem-cell renewal (PMID:24740926).

实验方案

Product Specific Protocols
WB protocol for JMJD5 antibody 20999-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols