验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HEK-293 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
产品信息
21572-1-AP targets JMJD4 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag16099 Product name: Recombinant human JMJD4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 114-463 aa of BC109272 Sequence: RRRWVTPAGRPDFDHLLRTYGDVVVPVANCGVQEYNSNPKEHMTLRDYITYWKEYIQAGYSSPRGCLYLKDWHLCRDFPVEDVFTLPVYFSSDWLNEFWDALDVDDYRFVYAGPAGSWSPFHADIFRSFSWSVNVCGRKKWLLFPPGQEEALRDRHGNLPYDVTSPALCDTHLHPRNQLAGPPLEITQEAGEMVFVPSGWHHQVHNLDDTISINHNWVNGFNLANMWRLLQQELCAVQEEVSEWRDSMPDWHHHCQVIMRSCSGINFEEFYHFLKVIAEKRLLVLREAAAEDGAGLGFEQAAFDVGRITEVLASLVAHPDFQRVDTSAFSPQPKELLQQLREAVDAAAAP 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | jumonji domain containing 4 |
| 别名 | JMJD4, jumonji domain containing 4 |
| 计算分子量 | 463 aa, 52 kDa |
| 观测分子量 | 48 kDa |
| GenBank蛋白编号 | BC109272 |
| 基因名称 | JMJD4 |
| Gene ID (NCBI) | 65094 |
| RRID | AB_2878886 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9H9V9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
JMJD4 (JmjC domain-containing protein 4, Jumonji domain-containing protein 4) is a member of the JmjC domain-containing proteins. Several JmjC domain-containing proteins have been reported to play a crucial role in the control of gene expression by acting as protein hydroxylases or histone demethylases (PMID: 16983801; 21300889). The function of JMJD4 protein remains unknown. The gene of JMJD4 maps to chromosome 1q42.13.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for JMJD4 antibody 21572-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Circulation Jmjd4 Facilitates Pkm2 Degradation in Cardiomyocytes and Is Protective Against Dilated Cardiomyopathy
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