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验证数据展示

  • WB analysis using 26637-1-AP

    WB analysis using 26637-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26637-1-AP (JMJD1C antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 26637-1-AP

    IF Staining of HeLa using 26637-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using JMJD1C antibody (26637-1-AP) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

JMJD1C Polyclonal antibody

JMJD1C Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 26637-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, ELISA

别名

TRIP8, TRIP 8, JHDM2C

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inU-251 cells, NIH/3T3 cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26637-1-AP targets JMJD1C in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag24348

Product name: Recombinant human JMJD1C protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 25-138 aa of BC143722

Sequence: TGIITHHDLFTRTMIVMNDQVLEPQNVDPSMVQMTFLDDVVHSLLKGENIGITSRRRSRANQNVNAVHSHYTRAQANSPRPAMNSQAAVPKQNTHQQQQQRSIRPNKRKGSDSS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 jumonji domain containing 1C
别名 TRIP8, TRIP 8, JHDM2C
计算分子量 2358 aa, 263 kDa
观测分子量285 kDa
GenBank蛋白编号BC143722
基因名称 JMJD1C
Gene ID (NCBI) 221037
RRIDAB_3669557
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ15652
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

JMJD1C, a member of the lysine demethylase 3 (KDM3) family, is universally required for the survival of several types of acute myeloid leukemia (AML) cells with different genetic mutations.

实验方案

Product Specific Protocols
IF protocol for JMJD1C antibody 26637-1-APDownload protocol
WB protocol for JMJD1C antibody 26637-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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