• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 32424-1-AP

    WB analysis using 32424-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32424-1-AP (JAM3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 32424-1-AP

    IHC staining of mouse testis using 32424-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 32424-1-AP (JAM3 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

JAM3 Polyclonal antibody

JAM3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 32424-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

JAM-2, JAM-3, JAM-C, Junctional adhesion molecule 3, Junctional adhesion molecule C

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inA375 cells, Jurkat cells, SH-SY5Y cells, SK-N-SH cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

32424-1-AP targets JAM3 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 JAM3 fusion protein Ag35750 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 junctional adhesion molecule 3
别名 JAM-2, JAM-3, JAM-C, Junctional adhesion molecule 3, Junctional adhesion molecule C
计算分子量35 kDa，310aa
观测分子量35 kDa
GenBank蛋白编号NM_032801.4
基因名称 JAM3
Gene ID (NCBI) 83700
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ9BX67
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Junctional adhesion molecule 3 (JAM3) is a type I transmembrane glycoprotein containing two Ig-like domains, which is expressed in various tissues and plays a crucial role in cell junctions, cell polarity, and motility (PMID: 34292449; 28931565). It has been proposed that JAM3 participates in leukocyte-platelet interactions, as well as angiogenesis and brain development (PMID: 12208882; 23255084). JAM3 may also be a new marker for predicting the prognosis and immune functions of bladder cancer patients (PMID: 38400838).

实验方案

Product Specific Protocols
WB protocol for JAM3 antibody 32424-1-APDownload protocol
IHC protocol for JAM3 antibody 32424-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols