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  • Featured Product
  • KD/KO Validated

JAKMIP1 Polyclonal antibody

JAKMIP1 Polyclonal Antibody for FC, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

FC, IHC, IP, WB,ELISA and More (1)

Conjugate

Unconjugated

Cat No : 13846-1-AP

Print datasheet

Synonyms

Gababrbp, JAKMIP1, JAMIP1, Marlin 1, MARLIN1

验证数据展示

  • WB analysis of mouse spleen using 13846-1-AP

    WB analysis of mouse spleen using 13846-1-AP

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 13846-1-AP (JAKMIP1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IP experiment of Jurkat using 13846-1-AP

    IP experiment of Jurkat using 13846-1-AP

    IP result of anti-JAKMIP1 (IP:13846-1-AP, 3ug; Detection:13846-1-AP 1:1000) with Jurkat cells lysate 4000ug.

  • IHC staining of human gliomas using 13846-1-AP

    IHC staining of human gliomas using 13846-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13846-1-AP (JAKMIP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human gliomas using 13846-1-AP

    IHC staining of human gliomas using 13846-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13846-1-AP (JAKMIP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of Jurkat using 13846-1-AP

    FC experiment of Jurkat using 13846-1-AP

    1X10^6 Jurkat cells were stained with 0.2ug JAKMIP1 antibody (13846-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

经过测试的应用

Positive WB detected inmouse spleen tissue, HEK-293 cells, Jurkat cells, mouse liver tissue, mouse testis tissue, Y79 cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inJurkat cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13846-1-AP targets JAKMIP1 in WB, IP, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications FC, IHC, IP, WB,ELISA
Cited ApplicationsWB, IF, IHC
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen JAKMIP1 fusion protein Ag4816 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name janus kinase and microtubule interacting protein 1
Synonyms Gababrbp, JAKMIP1, JAMIP1, Marlin 1, MARLIN1
Calculated Molecular Weight 73 kDa
Observed Molecular Weight 73 kDa
GenBank Accession NumberBC047075
Gene Symbol JAKMIP1
Gene ID (NCBI) 152789
RRIDAB_2128793
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96N16
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for JAKMIP1 antibody 13846-1-APDownload protocol
IHC protocol for JAKMIP1 antibody 13846-1-APDownload protocol
IP protocol for JAKMIP1 antibody 13846-1-APDownload protocol
FC protocol for JAKMIP1 antibody 13846-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IF

Neuron

JAKMIP1, a Novel Regulator of Neuronal Translation, Modulates Synaptic Function and Autistic-like Behaviors in Mouse.

Authors - Jamee M Berg
  • KO Validated
View Article
humanWB,IHC

Biomed Pharmacother

Overexpression of JAKMIP1 associates with Wnt/beta-catenin pathway activation and promotes cancer cell proliferation in vitro.

Authors - Okai Isaac I
View Article