验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HepG2 cells, MCF-7 cells, PC-3 cells, C6 cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
34193-1-AP targets JAGN1 in WB, IHC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag40789 Product name: Recombinant human JAGN1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-39 aa of BC032101 Sequence: MASRAGPRAAGTDGSDFQHRERVAMHYQMSVTLKYEIKK 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | jagunal homolog 1 (Drosophila) |
| 别名 | Protein jagunal homolog 1 |
| 计算分子量 | 183 aa, 21 kDa |
| 观测分子量 | 21 kDa |
| GenBank蛋白编号 | BC032101 |
| 基因名称 | JAGN1 |
| Gene ID (NCBI) | 84522 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q8N5M9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Jagunal homolog 1 (JAGN1) is an evolutionarily conserved endoplasmic reticulum (ER) membrane protein essential for neutrophil differentiation and function. First identified in Drosophila for its role in ER organization, human JAGN1 mutations cause severe congenital neutropenia type 5, characterized by recurrent infections due to impaired granulocyte development. JAGN1 regulates glycoprotein folding, ER-Golgi trafficking, and N-glycosylation of proteins critical for neutrophil viability. Loss of JAGN1 leads to ER stress, apoptosis of myeloid progenitors, and defective neutrophil migration. Its indispensable role in innate immunity highlights JAGN1 as a potential therapeutic target for neutropenia and immunodeficiency disorders (PMID: 25129144; 37120535; 33207009; 38739706).
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for JAGN1 antibody 34193-1-AP | Download protocol |
| WB protocol for JAGN1 antibody 34193-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
