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Species Reactivity
0
Species Reactivity
0
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Compatible Species
0
Conjugate
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验证数据展示

  • WB analysis using 83784-4-RR (same clone as 83784-4-PBS)

    WB analysis using 83784-4-RR (same clone as 83784-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83784-4-RR (Islet 1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83784-4-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 83784-4-RR (same clone as 83784-4-PBS)

    IHC staining of mouse brain using 83784-4-RR (same clone as 83784-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83784-4-RR (Islet 1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83784-4-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 83784-4-RR (same clone as 83784-4-PBS)

    IHC staining of mouse brain using 83784-4-RR (same clone as 83784-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83784-4-RR (Islet 1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83784-4-PBS in a different storage buffer formulation.

  • IF Staining of SH-SY5Y using 83784-4-RR (same clone as 83784-4-PBS)

    IF Staining of SH-SY5Y using 83784-4-RR (same clone as 83784-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using Islet 1 antibody (83784-4-RR, Clone: 240833D7 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83784-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83784-4-RR

    Affinity and Kinetic Characterization of 83784-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83784-4-RR against Human Islet 1 were performed. The affinity constant is below 1 pM.

Islet 1 Recombinant antibody, PBS Only

Islet 1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 83784-4-PBS

产品说明书

CloneNo. 240833D7

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

别名

ISL1, 240833D7, Insulin gene enhancer protein ISL-1, Isl 1, ISL LIM homeobox 1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

83784-4-PBS targets Islet 1 in WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 Islet 1 fusion protein Ag33541 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ISL LIM homeobox 1
别名 ISL1, 240833D7, Insulin gene enhancer protein ISL-1, Isl 1, ISL LIM homeobox 1
计算分子量 39 kDa
观测分子量 39-45 kDa
GenBank蛋白编号BC031213
基因名称 Islet 1
Gene ID (NCBI) 3670
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP61371
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ISL1 belongs to the LIM homeodomain transcription factor family that binds to the enhancer region of the INS gene. It plays a pivotal role in cardiogenesis, and lacking ISL1 expression results in growth arrest or displays profound defects in heart development, including atria, ventricle, and the inflow and outflow tracts. Ubiquitinated probably by WWP1 E3 ubiquitin ligase; ubiquitination is followed by protein degradation.