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验证数据展示

  • WB analysis using 28348-1-AP

    WB analysis using 28348-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28348-1-AP (Integrin alpha 9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Integrin alpha-9 Polyclonal antibody

Integrin alpha-9 Polyclonal Antibody for WB, ELISA
Cat No. 28348-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, Mouse

应用

WB, ELISA

别名

ALPHA RLC, Integrin alpha 9, Integrin alpha RLC, Integrin alpha-9, integrin, alpha 9, ITGA4L, ITGA9, RLC

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, NIH/3T3 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28348-1-AP targets Integrin alpha-9 in WB, ELISA applications and shows reactivity with Human, Mouse samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human, Mouse
免疫原 Integrin alpha-9 fusion protein Ag28815 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 integrin, alpha 9
别名 ALPHA RLC, Integrin alpha 9, Integrin alpha RLC, Integrin alpha-9, integrin, alpha 9, ITGA4L, ITGA9, RLC
计算分子量 114 kDa
观测分子量135 kDa
GenBank蛋白编号BC030198
基因名称 Integrin alpha 9
Gene ID (NCBI) 3680
RRIDAB_3669655
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ13797
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). Integrin alpha 9 contains a large N-terminal extracellular domain with 7 conserved repeats of putative metal-binding domains, a transmembrane segment, and a short C-terminal cytoplasmic tail (PMID: 8245132). Integrin alpha 9 interacts with integrin beta 1 generating α9β1 heterodimer, which is expressed in many cell types including epithelial cells, neutrophils, hepatocytes, muscle and endothelial cells (PMID: 21975548). It binds to a diversity of ligands in the extracellular matrix, like the a-disintegrin and metalloprotease (ADAM) family, THBS1, EMILIN1, VEGF, tenascin-C, osteopontin, fibronectin, and VCAM1 (PMID: 21975548; 33790909). Integrin α9β1 has been implicated in diverse biological functions including angiogenesis, lymphangiogenesis, lymphatic valve morphogenesis, granulopoiesis and tumorigenesis (PMID: 20179422).

实验方案

Product Specific Protocols
WB protocol for Integrin alpha-9 antibody 28348-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols