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验证数据展示

  • WB analysis using 12594-1-AP (same clone as 12594-1-PBS)

    WB analysis using 12594-1-AP (same clone as 12594-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12594-1-AP (Integrin Beta 1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12594-1-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 12594-1-AP (same clone as 12594-1-PBS)

    IHC staining of human placenta using 12594-1-AP (same clone as 12594-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 12594-1-AP (Integrin Beta 1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12594-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ITGB1/CD29 antibody included
  • IHC staining of human placenta using 12594-1-AP (same clone as 12594-1-PBS)

    IHC staining of human placenta using 12594-1-AP (same clone as 12594-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 12594-1-AP (Integrin Beta 1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12594-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ITGB1/CD29 antibody included
  • IF Staining of A431 using 12594-1-AP (same clone as 12594-1-PBS)

    IF Staining of A431 using 12594-1-AP (same clone as 12594-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using Integrin Beta 1 antibody (12594-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 12594-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 12594-1-AP (same clone as 12594-1-PBS)

    IF Staining of HeLa using 12594-1-AP (same clone as 12594-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Integrin Beta 1 antibody (12594-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 12594-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

Integrin Beta 1 Polyclonal antibody, PBS Only

Integrin Beta 1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 12594-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

ITGB1, CD29, FNRB, Glycoprotein IIa, GPIIA

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy ITGB1/CD29 Ready-To-Use IHC Kit. ITGB1/CD29 primary antibody included.

产品信息

12594-1-PBS targets Integrin Beta 1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Integrin Beta 1 fusion protein Ag3078 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12)
别名 ITGB1, CD29, FNRB, Glycoprotein IIa, GPIIA
计算分子量 88 kDa
观测分子量 100-130 kDa
GenBank蛋白编号BC020057
基因名称 Integrin beta 1
Gene ID (NCBI) 3688
ENSEMBL Gene IDENSG00000150093
RRIDAB_2130085
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP05556
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Integrin beta-1 (ITGB1), also named as CD29, is a single chain type I glycoprotein that is expressed in a heterodimeric complex with one of six distinct α subunits, comprising the very late activation antigen (VLA) subfamily of adhesion receptors. It is one of the essential surface molecules expressed on human MSC from bone marrow and other sources. The β1 subunit is also broadly expressed on lymphocytes and monocytes, weakly expressed on granulocytes, and not expressed on erythrocytes. These receptors are involved in a variety of cell-cell and cell-matrix interactions.