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验证数据展示

  • WB analysis of A549 using 66070-1-Ig (same clone as 66070-1-PBS)

    WB analysis of A549 using 66070-1-Ig (same clone as 66070-1-PBS)

    WB result of Integrin Alpha 3 antibody (66070-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Integrin Alpha 3 transfected A549 cells. This data was developed using the same antibody clone with 66070-1-PBS in a different storage buffer formulation.

  • WB analysis of PC-3 using 66070-1-Ig (same clone as 66070-1-PBS)

    WB analysis of PC-3 using 66070-1-Ig (same clone as 66070-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 66070-1-Ig (ITGA3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66070-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 66070-1-Ig (same clone as 66070-1-PBS)

    IHC staining of human lung cancer using 66070-1-Ig (same clone as 66070-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66070-1-Ig (Integrin alpha-3 antibody) at dilution of 1:4000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66070-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ITGA3 antibody included
  • IHC staining of human lung cancer using 66070-1-Ig (same clone as 66070-1-PBS)

    IHC staining of human lung cancer using 66070-1-Ig (same clone as 66070-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66070-1-Ig (Integrin alpha-3 antibody) at dilution of 1:4000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66070-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ITGA3 antibody included
  • Featured Product
  • KD/KO验证

Integrin Alpha 3 Monoclonal antibody, PBS Only

Integrin Alpha 3 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66070-1-PBS

产品说明书

CloneNo. 3F9G4

宿主/亚型

Mouse / IgG2a

种属反应性

human, pig, mouse

应用

WB, IHC, Indirect ELISA

别名

CD49C, FRP 2, Galactoprotein B3, GAP B3, GAPB3, Integrin alpha 3, ITGA3, MSK18, VCA 2, VL3A, VLA 3 subunit alpha, VLA3a

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy ITGA3 Ready-To-Use IHC Kit. ITGA3 primary antibody included.

产品信息

66070-1-PBS targets Integrin Alpha 3 in WB, IHC, Indirect ELISA applications and shows reactivity with human, pig, mouse samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, pig, mouse
免疫原 Integrin Alpha 3 fusion protein Ag17293 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 integrin, alpha 3 (antigen CD49C, alpha 3 subunit of VLA-3 receptor)
别名 CD49C, FRP 2, Galactoprotein B3, GAP B3, GAPB3, Integrin alpha 3, ITGA3, MSK18, VCA 2, VL3A, VLA 3 subunit alpha, VLA3a
计算分子量 1066 aa, 119 kDa
观测分子量 120 kDa
GenBank蛋白编号BC150190
基因名称 Integrin alpha 3
Gene ID (NCBI) 3675
RRIDAB_11042765
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP26006
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ITGA3, also named as MSK18, CD49c, GAPB3 and FRP-2, belongs to the integrin alpha chain family. It is a receptor for fibronectin, laminin, collagen, epiligrin, thrombospondin and CSPG4. ITGA3 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration. ITGA3 is highly concentrated in epithelial cells where it strongly adheres to Laminin-5 and Laminin-5 induced rapid adhesion can be blocked by antibodies against the alpha-3 integrin subunit. It can be cleaved into heavy chain(840aa) and light chain(176aa). This antibody can recognize the heavy chain.