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验证数据展示

  • WB analysis using 86630-3-RR

    WB analysis using 86630-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86630-3-RR (KPNB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 86630-3-RR

    WB analysis of HeLa using 86630-3-RR

    WB result of Importin Beta antibody (86630-3-RR; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Importin Beta transfected HeLa cells.

  • Affinity and Kinetic Characterization of 86630-3-RR

    Affinity and Kinetic Characterization of 86630-3-RR

    Biolayer interferometry (BLl) kinetic assays of 86630-3-RR against Human Importin Beta were performed. The affinity constant is 1.02 nM.

  • Featured Product
  • KD/KO Validated

Importin Beta Recombinant monoclonal antibody

Importin Beta Uni-rAb® Recombinant Antibody for WB, ELISA
Cat No. 86630-3-RR

产品说明书

CloneNo. 251633D12

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, ELISA

别名

KPNB1, IMB1, Impnb, Importin 90, Importin subunit beta 1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA2780 cells, HeLa cells, HT-1080 cells, NIH/3T3 cells, C6 cells, mouse brain tissue, rat brain tissue
Planning an IHC experiment? We recommend our IHCeasy KPNB1 Ready-To-Use IHC Kit. KPNB1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86630-3-RR targets Importin Beta in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag0122

Product name: Recombinant human KPNB1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 409-612 aa of BC003572

Sequence: AMPTLIELMKDPSVVVRDTAAWTVGRICELLPEAAINDVYLAPLLQCLIEGLSAEPRVASNVCWAFSSLAEAAYEAADVADDQEEPATYCLSSSFELIVQKLLETTDRPDGHQNNLRSSAYESLMEIVKNSAKDCYPAVQKTTLVIMERLQQVLQMESHIQSTSDRIQFNDLQSLLCATLQNVLRKVQHQDALQISDVVMASLL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 karyopherin (importin) beta 1
别名 KPNB1, IMB1, Impnb, Importin 90, Importin subunit beta 1
计算分子量 97 kDa
观测分子量 90-97 kDa
GenBank蛋白编号BC003572
基因名称 KPNB1
Gene ID (NCBI) 3837
RRIDAB_3745020
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ14974
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Importin beta1, also known karyopherin beta or KPNB1, is a nuclear transport receptor that plays a key role in the nuclear import process. Importin beta1 and importin alpha work in concert to transport cargo into the nucleus, functioning as an essential component of the classical nuclear localization signal (NLS) pathway. Elevated expression of importin beta1 has been found in cervical tumors. (22125623)

实验方案

Product Specific Protocols
WB protocol for Importin Beta antibody 86630-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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