验证数据展示
经过测试的应用
Positive IHC detected in | mouse spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
IHC | See 1 publications below |
产品信息
19538-1-AP targets CXCR2 in IHC, ELISA applications and shows reactivity with mouse samples.
经测试应用 | IHC, ELISA Application Description |
文献引用应用 | IHC |
经测试反应性 | mouse |
文献引用反应性 | mouse |
免疫原 | Peptide 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | interleukin 8 receptor, beta |
别名 | Cxcr2-Specific, CXCR-2, CXC-R2, C-X-C chemokine receptor type 2, Cmkar2 |
计算分子量 | 41 kDa |
GenBank蛋白编号 | NM_009909 |
基因名称 | Cxcr2 |
Gene ID (NCBI) | 12765 |
RRID | AB_2878587 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P35343 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CXCR2, also named as Cmkar2, Gpcr16, Il8rb or CD182, belongs to the G-protein coupled receptor 1 family. CXCR2 is a receptor for interleukin-8 which is a powerful neutrophil chemotactic factor. Binding of IL-8 to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system. CXCR2 binds to IL-8 with high affinity. CXCR2 also binds with high affinity to CXCL3, GRO/MGSA and NAP-2. This antibody is specific to mouse CXCR2.
实验方案
Product Specific Protocols | |
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IHC protocol for CXCR2 antibody 19538-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |