• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of PC-3 using 51066-1-AP (same clone as 51066-1-PBS)

    WB analysis of PC-3 using 51066-1-AP (same clone as 51066-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 51066-1-AP (IkB alpha antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51066-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 51066-1-AP (same clone as 51066-1-PBS)

    IP experiment of HeLa using 51066-1-AP (same clone as 51066-1-PBS)

    IP result of anti-IkB Alpha (IP:51066-1-AP, 4ug; Detection:51066-1-AP 1:1000) with HeLa cells lysate 3200ug. This data was developed using the same antibody clone with 51066-1-PBS in a different storage buffer formulation.

  • IHC staining of human cervical cancer using 51066-1-AP (same clone as 51066-1-PBS)

    IHC staining of human cervical cancer using 51066-1-AP (same clone as 51066-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 51066-1-AP (IkB alpha antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 51066-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NFKBIA antibody included
  • IHC staining of human cervical cancer using 51066-1-AP (same clone as 51066-1-PBS)

    IHC staining of human cervical cancer using 51066-1-AP (same clone as 51066-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 51066-1-AP (IkB alpha antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 51066-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NFKBIA antibody included

IkB Alpha Polyclonal antibody, PBS Only

IkB Alpha Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 51066-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IP, Indirect ELISA

别名

IKBA, IκB α, NFKBIA, I kappa B alpha, IkappaBalpha

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

Planning an IHC experiment? We recommend our IHCeasy NFKBIA Ready-To-Use IHC Kit. NFKBIA primary antibody included.

产品信息

51066-1-PBS targets IkB Alpha in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
别名 IKBA, IκB α, NFKBIA, I kappa B alpha, IkappaBalpha
计算分子量 36 kDa
观测分子量 39 kDa
GenBank蛋白编号BC002601
基因名称 IkB Alpha
Gene ID (NCBI) 4792
RRIDAB_2235946
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP25963
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (NFKBIA, synonyms: IKBA, MAD-3, NFKBI). NFKB1 or NFKB2 is bound to REL, RELA , or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime.

||
New chat Able
正在加载，请稍候...