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1X10^6 mouse splenocytes were surface stained with 0.5 ug Anti-Armenian Hamster IgG Isotype Control (65210-1-Ig, Clone: PIP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:200 (black), or stained with 0.5 ug Anti-Mouse CD3ε (65060-1-Ig, Clone: 145-2C11) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:200 (green). Cells were not fixed.
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
The PIP immunoglobulin is useful as an isotype-matched control. The PIP immunolglobulin is specific for Glutathione S-transferase and can be used as an isotype control for Armenian Hamster IgG antibodies. This antibody has been quality-tested for flow cytometry as negative control.