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验证数据展示

  • WB analysis of HEK-293 using 25964-1-AP

    WB analysis of HEK-293 using 25964-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 25964-1-AP (Icln antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HEK-293 using 25964-1-AP

    IF Staining of HEK-293 using 25964-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 25964-1-AP (Icln antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Icln Polyclonal antibody

Icln Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 25964-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, Canine

应用

WB, IF/ICC, ELISA

别名

CLCI, CLNS1A, CLNS1B, I(Cln), ICln, Methylosome subunit pICln, pICln, Reticulocyte pICln

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, MDCK cells
Positive IF/ICC detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy CLNS1A Ready-To-Use IHC Kit. CLNS1A primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25964-1-AP targets Icln in WB, IF/ICC, ELISA applications and shows reactivity with human, Canine samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, Canine
免疫原

CatNo: Ag22923

Product name: Recombinant human CLNS1A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-237 aa of BC119634

Sequence: MSFLKSFPPPGPAEGLLRQQPDTEAVLNGKGLGTGTLYIAESRLSWLDGSGLGFSLEYPTISLHALSRDRSDCLGEHLYVMVNAKFEEESKEPVADEEEEDSDDDVEPITEFRFVPSDKSALEAMFTAMCECQALHPDPEDEDSDDYDGEEYDVEAHEQGQGDIPTFYTYEEGLSHLTAEGQATLERLEGMLSQSVSSQYNMAGVRTEDSIRDYEDGMEVDTTPTVAGQFEDADVDH

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chloride channel, nucleotide-sensitive, 1A
别名 CLCI, CLNS1A, CLNS1B, I(Cln), ICln, Methylosome subunit pICln, pICln, Reticulocyte pICln
计算分子量 237 aa, 26 kDa
观测分子量 36-42 kDa
GenBank蛋白编号BC119634
基因名称 CLNS1A
Gene ID (NCBI) 1207
RRIDAB_2880312
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP54105
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for Icln antibody 25964-1-APDownload protocol
WB protocol for Icln antibody 25964-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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