验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, MDCK cells |
| Positive IF/ICC detected in | HEK-293 cells |
Planning an IHC experiment? We recommend our IHCeasy CLNS1A Ready-To-Use IHC Kit. CLNS1A primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
25964-1-AP targets Icln in WB, IF/ICC, ELISA applications and shows reactivity with human, Canine samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, Canine |
| 免疫原 |
CatNo: Ag22923 Product name: Recombinant human CLNS1A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-237 aa of BC119634 Sequence: MSFLKSFPPPGPAEGLLRQQPDTEAVLNGKGLGTGTLYIAESRLSWLDGSGLGFSLEYPTISLHALSRDRSDCLGEHLYVMVNAKFEEESKEPVADEEEEDSDDDVEPITEFRFVPSDKSALEAMFTAMCECQALHPDPEDEDSDDYDGEEYDVEAHEQGQGDIPTFYTYEEGLSHLTAEGQATLERLEGMLSQSVSSQYNMAGVRTEDSIRDYEDGMEVDTTPTVAGQFEDADVDH 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | chloride channel, nucleotide-sensitive, 1A |
| 别名 | CLCI, CLNS1A, CLNS1B, I(Cln), ICln, Methylosome subunit pICln, pICln, Reticulocyte pICln |
| 计算分子量 | 237 aa, 26 kDa |
| 观测分子量 | 36-42 kDa |
| GenBank蛋白编号 | BC119634 |
| 基因名称 | CLNS1A |
| Gene ID (NCBI) | 1207 |
| RRID | AB_2880312 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P54105 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for Icln antibody 25964-1-AP | Download protocol |
| WB protocol for Icln antibody 25964-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
