验证数据展示
经过测试的应用
| Positive WB detected in | fetal human brain tissue |
| Positive IHC detected in | human stomach tissue, human intrahepatic cholangiocarcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
21862-1-AP targets Intersectin 1 in WB, IHC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 | Intersectin 1 fusion protein Ag16517 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | intersectin 1 (SH3 domain protein) |
| 别名 | ITSN1, SH3 domain-containing protein 1A, ITSN 1, ITSN, Intersectin-1 |
| 计算分子量 | 1721 aa, 195 kDa |
| 观测分子量 | 150 kDa |
| GenBank蛋白编号 | BC116186 |
| 基因名称 | Intersectin 1 |
| Gene ID (NCBI) | 6453 |
| RRID | AB_2878931 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q15811 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Intersectin 1 (ITSN1) is a multidomain adaptor protein that plays a crucial role in various cellular processes, particularly in the nervous system. It is involved in clathrin-mediated endocytosis, signal transduction, and the regulation of the actin cytoskeleton. The protein is highly abundant in neurons and has been implicated in neurodevelopmental disorders, such as autism spectrum disorders, intellectual disability, and epilepsy. Recent studies have identified de novo variants in the ITSN1 gene in patients with these disorders, suggesting a link between ITSN1 and brain development.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Intersectin 1 antibody 21862-1-AP | Download protocol |
| IHC protocol for Intersectin 1 antibody 21862-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
