验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, MDA-MB-453s cells |
| Positive IP detected in | A549 cells, HuH-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
26055-1-AP targets ITPRIP in WB, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag22012 Product name: Recombinant human ITPRIP protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 201-547 aa of BC070108 Sequence: QVDRPLLCHLFVPFTPPEPYRFHPELWCSGRSVPLDRQGYGQIKVVRADGDTLSCICGKTKLGEDMLCLLHGRNSMAPPCGDMENLLCATDSLYLDTMQVMKWFQTALTRAWKGIAHKYEFDLAFGQLDSPGSLKIKFRSGKFMPFNLIPVIQCDDSDLYFVSHLPREPSEGTPASSTDWLLSFAVYERHFLRTTLKALPEGACHLSCLQIASFLLSKQSRLTGPSGLSSYHLKTALLHLLLLRQAADWKAGQLDARLHELLCFLEKSLLQKKLHHFFIGNRKVPEAMGLPEAVLRAEPLNLFRPFVLQRSLYRKTLDSFYEMLKNAPALISEYSLHVPSDQPTPKS 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | inositol 1,4,5-triphosphate receptor interacting protein |
| 别名 | bA127L20, DANGER, Inositol 1,4,5-trisphosphate receptor-interacting protein, KIAA1754, Protein DANGER |
| 计算分子量 | 547 aa, 62 kDa |
| 观测分子量 | 62 kDa |
| GenBank蛋白编号 | BC070108 |
| 基因名称 | ITPRIP |
| Gene ID (NCBI) | 85450 |
| RRID | AB_2880354 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8IWB1 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ITPRIP (inositol 1,4,5-trisphosphate receptor interacting protein) is a key regulatory protein that binds to IP3 receptors to modulate endoplasmic reticulum calcium release and intracellular calcium homeostasis. It is widely expressed in human tissues with high abundance in the central nervous system. ITPRIP participates in neurodevelopment, cell apoptosis, innate immunity and tumor progression. Its abnormal expression is closely linked to glioma malignancy, neurodevelopmental disorders and immune dysfunction, serving as an essential molecule in physiological regulation and disease pathogenesis. (PMID: 29899107)
实验方案
| Product Specific Protocols | |
|---|---|
| IP protocol for ITPRIP antibody 26055-1-AP | Download protocol |
| WB protocol for ITPRIP antibody 26055-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
