验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells |
| Positive IHC detected in | mouse colon tissue, mouse small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
产品信息
20729-1-AP targets ITPR3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | inositol 1,4,5-triphosphate receptor, type 3 |
| 别名 | InsP3R3, IP3 receptor isoform 3, IP3R, IP3R 3, IP3R3, ITPR3, Type 3 InsP3 receptor |
| 计算分子量 | 304 kDa |
| 观测分子量 | 250-300 kDa |
| GenBank蛋白编号 | NM_002224 |
| 基因名称 | ITPR3 |
| Gene ID (NCBI) | 3710 |
| RRID | AB_3669343 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q14573 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ITPRs family have three members: ITPR1, ITPR2 and ITPR3. For ITPR3, it is associated with cervical squamous cell carcinoma, glioblastoma, cholangiocarcinoma and other cancers. Inositol 1,4, 5-triphosphate receptor type 3 (ITPR3) was found to be apical-located in bile duct cells, and disruption of lipid raft in the segregated bile duct unit (IBDU) redistributes ITPR3 and impairs Ca2+ waves. (PMID: 35580861, PMID:35852334)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ITPR3 antibody 20729-1-AP | Download protocol |
| IHC protocol for ITPR3 antibody 20729-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Ethnopharmacol Zhizichi decoction alleviates depressive-like behaviors through modulating mitochondria-associated membrane via the IP3R3-GRP75-VDAC1 complex | ||
Mol Cell Endocrinol High glucose elevates intracellular calcium level and induces ferroptosis in glomerular endothelial cells through the miR-223-3p/ITPR3 pathway |
