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验证数据展示

  • WB analysis using 20729-1-AP

    WB analysis using 20729-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20729-1-AP (ITPR3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse colon using 20729-1-AP

    IHC staining of mouse colon using 20729-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 20729-1-AP (ITPR3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ITPR3 Polyclonal antibody

ITPR3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 20729-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

InsP3R3, IP3 receptor isoform 3, IP3R, IP3R 3, IP3R3, ITPR3, Type 3 InsP3 receptor

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells
Positive IHC detected inmouse colon tissue, mouse small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

20729-1-AP targets ITPR3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 inositol 1,4,5-triphosphate receptor, type 3
别名 InsP3R3, IP3 receptor isoform 3, IP3R, IP3R 3, IP3R3, ITPR3, Type 3 InsP3 receptor
计算分子量 304 kDa
观测分子量250-300 kDa
GenBank蛋白编号NM_002224
基因名称 ITPR3
Gene ID (NCBI) 3710
RRIDAB_3669343
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ14573
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ITPRs family have three members: ITPR1, ITPR2 and ITPR3. For ITPR3, it is associated with cervical squamous cell carcinoma, glioblastoma, cholangiocarcinoma and other cancers. Inositol 1,4, 5-triphosphate receptor type 3 (ITPR3) was found to be apical-located in bile duct cells, and disruption of lipid raft in the segregated bile duct unit (IBDU) redistributes ITPR3 and impairs Ca2+ waves. (PMID: 35580861, PMID:35852334)

实验方案

Product Specific Protocols
WB protocol for ITPR3 antibody 20729-1-APDownload protocol
IHC protocol for ITPR3 antibody 20729-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Cell Endocrinol

High glucose elevates intracellular calcium level and induces ferroptosis in glomerular endothelial cells through the miR-223-3p/ITPR3 pathway

Authors - Dekai Wang
View Article