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验证数据展示

  • WB analysis using 85101-1-RR

    WB analysis using 85101-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85101-1-RR (ITPR1-specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 85101-1-RR

    IHC staining of mouse brain using 85101-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85101-1-RR (ITPR1-Specific antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 85101-1-RR

    IHC staining of mouse brain using 85101-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85101-1-RR (ITPR1-Specific antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ITPR1-Specific Recombinant monoclonal antibody

ITPR1-Specific Uni-rAb® Recombinant Antibody for WB, IHC, ELISA
Cat No. 85101-1-RR

产品说明书

CloneNo. 242274B10

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

ITPR1, 242274B10, ACV, Inositol 1,4,5 trisphosphate receptor, Inositol 1,4,5-trisphosphate receptor type 1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inSH-SY5Y cells, HeLa cells, L02 cells, mouse brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85101-1-RR targets ITPR1-Specific in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 inositol 1,4,5-triphosphate receptor, type 1
别名 ITPR1, 242274B10, ACV, Inositol 1,4,5 trisphosphate receptor, Inositol 1,4,5-trisphosphate receptor type 1
计算分子量 314 kDa
观测分子量 290-300 kDa
GenBank蛋白编号NM_001099952
基因名称 ITPR1
Gene ID (NCBI) 3708
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ14643
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ITPR1, also named as INSP3R1 and IP3R, belongs to the InsP3 receptor family. It is a receptor for inositol 1,4,5-trisphosphate which is a second messenger that mediates the release of intracellular calcium. Defects in ITPR1 are the cause of spinocerebellar ataxia type 15 (SCA15). The antibody has no cross reaction with ITPR2 and ITPR3.

实验方案

Product Specific Protocols
IHC protocol for ITPR1-Specific antibody 85101-1-RRDownload protocol
WB protocol for ITPR1-Specific antibody 85101-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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