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ITPK1 Polyclonal antibody

ITPK1 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 10568-1-AP

Print datasheet

Synonyms

Ins(1,3,4)P(3) 5/6 kinase, ITPK1, ITRPK1

验证数据展示

  • IHC staining of human brain using 10568-1-AP

    IHC staining of human brain using 10568-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10568-1-AP (ITPK1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human brain using 10568-1-AP

    IHC staining of human brain using 10568-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10568-1-AP (ITPK1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of human brain using 10568-1-AP

    WB analysis of human brain using 10568-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 10568-1-AP (ITPK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inhuman brain tissue, HEK-293 cells, mouse eye tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10568-1-AP targets ITPK1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, WB,ELISA
Tested Reactivity human, mouse
Immunogen ITPK1 fusion protein Ag0834 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name inositol 1,3,4-triphosphate 5/6 kinase
Synonyms Ins(1,3,4)P(3) 5/6 kinase, ITPK1, ITRPK1
Calculated Molecular Weight 48 kDa
Observed Molecular Weight 45 kDa
GenBank Accession NumberBC007428
Gene Symbol ITPK1
Gene ID (NCBI) 3705
RRIDAB_2129832
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13572
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ITPK1 antibody 10568-1-APDownload protocol
IHC protocol for ITPK1 antibody 10568-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols