验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, HepG2 cells, Jurkat cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
18306-1-AP targets ITM2A in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IF, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | integral membrane protein 2A |
| 别名 | BRICD2A, E25A, integral membrane protein 2A, ITM2A, Protein E25 |
| 计算分子量 | 30 kDa |
| 观测分子量 | 32 kDa |
| GenBank蛋白编号 | BC040437 |
| 基因名称 | ITM2A |
| Gene ID (NCBI) | 9452 |
| RRID | AB_2249412 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O43736 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ITM2A, also named BRICD2A and E25A, is a member of type II integral membrane (ITM2) protein family. ITM2A expression is high in osteogenic and lymphoid tissues, while both ITM2B and ITM2C are expressed in brain. There are 5 articles specifically referring to this gene in PubMed. Proteins are expected to localize in various compartments (cytoplasm, integral to membrane). No phenotype has yet been reported to our knowledge: this gene's in vivo function is yet unknown.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ITM2A antibody 18306-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Bone Res Single cell analysis reveals inhibition of angiogenesis attenuates the progression of heterotopic ossification in Mkx-/- mice. | ||
Blood Identification of early gene expression changes during human Th17 cell differentiation. | ||
Cell Commun Signal Integral membrane protein 2A inhibits cell growth in human breast cancer via enhancing autophagy induction. | ||
iScience The regenerating skeletal muscle niche drives satellite cell return to quiescence. | ||
PLoS One Itm2a expression in the developing mouse first lower molar, and the subcellular localization of itm2a in mouse dental epithelial cells. |