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验证数据展示

  • WB analysis using 18815-1-AP

    WB analysis using 18815-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18815-1-AP (ITLN2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 18815-1-AP

    IHC staining of human colon cancer using 18815-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 18815-1-AP (ITLN2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ITLN2 Polyclonal antibody

ITLN2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 18815-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

WB, IHC, ELISA

别名

Intelectin-2, intelectin 2, HL2, HL 2, Endothelial lectin HL-2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse small intestine tissue, mouse colon tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18815-1-AP targets ITLN2 in WB, IHC, ELISA applications and shows reactivity with mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 intelectin 2
别名 Intelectin-2, intelectin 2, HL2, HL 2, Endothelial lectin HL-2
计算分子量 36 kDa
观测分子量36 kDa
GenBank蛋白编号BC143341
基因名称 ITLN2
Gene ID (NCBI) 142683
RRIDAB_3669302
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8WWU7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ITLN2 also named as UNQ2789 and PRO7179, may play a role in the defense system against pathogens. Intelectins (intestinal lectins) are highly conserved across chordate evolution and have been implicated in various human diseases, including Crohn's disease (CD). The human genome encodes two intelectin genes, intelectin-1 (ITLN1) and intelectin-2 (ITLN2). It is reproted that human ITLN2 is expressed in Paneth cells, where it localizes to secretory granules, and establish that ITLN2 is a highly abundant multimeric protein secreted into the lumen of the small intestine (PMID: 35182405). The calculated molecular weight of ITLN2 is 36 kDa.

实验方案

Product Specific Protocols
WB protocol for ITLN2 antibody 18815-1-APDownload protocol
IHC protocol for ITLN2 antibody 18815-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols