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ITK Monoclonal antibody

ITK Monoclonal Antibody for FC, IF/ICC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

FC, IF/ICC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1F9D8

Cat No : 67703-1-Ig

Print datasheet

Synonyms

EMT, IL2 inducible T cell kinase, ITK, Kinase EMT, LYK, PSCTK2, T cell specific kinase, Tyrosine protein kinase Lyk

验证数据展示

  • WB analysis of PBMC using 67703-1-Ig

    WB analysis of PBMC using 67703-1-Ig

    PBMC cells were subjected to SDS PAGE followed by western blot with 67703-1-Ig (ITK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of Jurkat using 67703-1-Ig

    WB analysis of Jurkat using 67703-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67703-1-Ig (ITK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • FC experiment of Jurkat using 67703-1-Ig

    FC experiment of Jurkat using 67703-1-Ig

    1X10^6 Jurkat cells were intracellularly stained with 0.2 ug Anti-Human ITK (67703-1-Ig, Clone:1F9D8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Mouse IgG2a Isotype Control (66360-2-Ig, Clone: K11A1B2A2) (black). Cells were fixed with 90% MeOH .

  • IF Staining of Jurkat using 67703-1-Ig

    IF Staining of Jurkat using 67703-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using ITK antibody (67703-1-Ig, Clone: 1F9D8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inJurkat cells, PBMC cells
Positive IF detected inJurkat cells
Positive FC detected inJurkat cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67703-1-Ig targets ITK in WB, IF, FC, ELISA applications and shows reactivity with Human samples.

Tested Applications FC, IF/ICC, WB, ELISA
Tested Reactivity Human
Immunogen ITK fusion protein Ag17087 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name IL2-inducible T-cell kinase
Synonyms EMT, IL2 inducible T cell kinase, ITK, Kinase EMT, LYK, PSCTK2, T cell specific kinase, Tyrosine protein kinase Lyk
Calculated Molecular Weight 620 aa, 72 kDa
Observed Molecular Weight70 kDa
GenBank Accession NumberBC109077
Gene Symbol ITK
Gene ID (NCBI) 3702
RRIDAB_2882895
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ08881
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

ITK is a member of Tec family (BTK, ITK, Tec, BMX and RLK), which expressed in normal T-lymphocytes and T-cell associated hematopoietic malignancies and have confirmed its critical role in regulating T lymphocyte function in EBV-driven lymphoproliferative disease and immune-mediated disorders. Tec kinase family members shares similarities structure, consisting of PH domain, SH3 domain, SH2 domain and kinase domain. Loss of function mutations in ITK results in hypogammaglobulinemia and CD4+ T cell loss in humans, and the patients often present with EBV-associated B cell lymphoproliferative syndrome (PMID: 30814910, PMID: 31025232).

实验方案

Product Specific Protocols
WB protocol for ITK antibody 67703-1-IgDownload protocol
IF protocol for ITK antibody 67703-1-IgDownload protocol
FC protocol for ITK antibody 67703-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols