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ITK Polyclonal antibody

ITK Polyclonal Antibody for FC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC, WB, ELISA

Conjugate

Unconjugated

Cat No : 21525-1-AP

Print datasheet

Synonyms

EMT, IL2 inducible T cell kinase, ITK, Kinase EMT, LYK, PSCTK2, T cell specific kinase, Tyrosine protein kinase Lyk

验证数据展示

  • WB analysis using 21525-1-AP

    WB analysis using 21525-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21525-1-AP (ITK antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • FC experiment of Jurkat using 21525-1-AP

    FC experiment of Jurkat using 21525-1-AP

    1X10^6 Jurkat cells were intracellularly stained with 0.5 ug Anti-Human ITK (21525-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.5 ug Control Antibody. Cells were fixed with 90% MeOH .

经过测试的应用

Positive WB detected inJurkat cells, mouse spleen tissue
Positive FC detected inJurkat cells
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®555 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Flow Cytometry (FC)FC : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

21525-1-AP targets ITK in WB, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen ITK fusion protein Ag16132 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name IL2-inducible T-cell kinase
Synonyms EMT, IL2 inducible T cell kinase, ITK, Kinase EMT, LYK, PSCTK2, T cell specific kinase, Tyrosine protein kinase Lyk
Calculated Molecular Weight 620 aa, 72 kDa
Observed Molecular Weight 68-72 kDa
GenBank Accession NumberBC109077
Gene Symbol ITK
Gene ID (NCBI) 3702
RRIDAB_10733884
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ08881
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ITK antibody 21525-1-APDownload protocol
FC protocol for ITK antibody 21525-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cytotherapy

Bruton tyrosine kinase inhibitors preserve anti-CD19 chimeric antigen receptor T-cell functionality and reprogram tumor micro-environment in B-cell lymphoma

Authors - Wenjing Luo
View Article